Binette J P, Binette M B
Veterans Administration Medical Center, Buffalo 14215.
Scanning Microsc. 1994;8(2):233-9.
Proteins from urinary tract and gallbladder stones were extracted and characterized to determine the composition of the matrix and possibly unravel the role of the organic phase in stone formation. Proteins from crushed stones were extracted by electrodialysis and concentrated in the Amicon centricon cartridge or by lyophilization after dialysis against distilled water. Aliquots were first analyzed by isoelectric focusing in gel and if suitable subjected to two-dimensional (2D) electrophoresis. The most promising spots were harvested and the N-terminal amino acids sequenced, thus providing maximum information with minimum expenditure of material. The 2D separations and amino acid sequences of several protein extracts demonstrated similarities and differences in composition and achieved the identification or demonstration of previously and recently detected polypeptides.
提取并表征了来自尿路结石和胆囊结石的蛋白质，以确定基质的组成，并有可能阐明有机相在结石形成中的作用。通过电渗析从碎石中提取蛋白质，并在Amicon离心过滤柱中浓缩，或在对蒸馏水透析后冻干。首先通过凝胶等电聚焦分析等分试样，若合适则进行二维（2D）电泳。收集最有前景的斑点并对N端氨基酸进行测序，从而以最少的材料消耗提供最大的信息量。几种蛋白质提取物的二维分离和氨基酸序列显示出组成上的异同，并实现了对先前和最近检测到的多肽的鉴定或证明。
