Ahmed A A, Nordlind K, Schultzberg M, Lidén S
Department of Dermatology, Karolinska Hospital, Stockholm, Sweden.
Acta Derm Venereol. 1994 Nov;74(6):435-40. doi: 10.2340/0001555574435440.
Paraformaldehyde-fixed tissue of chronic granulomatous skin conditions, such as cutaneous leishmaniasis, granuloma annulare, leprosy and hidroadenitis, was investigated for the presence of interleukin-1 alpha-, interleukin-1 beta-, interleukin-6- and tumour necrosis factor-alpha-like immunoreactivities among the cellular infiltrates. There was a weak to strong cytoplasmic labelling of plasma cells for interleukin-6 and tumour necrosis factor-alpha at the periphery of the granulomatous mass and around the skin appendages. The interleukin-6-like immunoreactivity seemed to be correlated with the coarseness of the chromatin material of the cells, being more intense with coarse chromatin. The cytoplasmic labelling for interleukin-1 alpha and interleukin-1 beta in the plasma cells was less intense. Epitheloid, Langhans' giant cells and small round cells exhibited a weak to moderate cytoplasmic labelling for interleukin-1 alpha and interleukin-1 beta, whereas the staining intensity for interleukin-6 and tumour necrosis factor-alpha was weak to strong. In addition, there was staining of the stroma in the centre of granuloma with antisera against interleukin-1 alpha, interleukin-1 beta, interleukin-6 and tumour necrosis factor-alpha. This area contained few cells, suggesting that the granuloma was in a resolution process. A contribution of interleukin-6 and tumour necrosis factor-alpha to the granulomatous reaction, at least during the maintenance period, is suggested by the occurrence of these cytokines in the skin conditions studied. The findings are also consistent with a suggested role of B cells in the late stages of the granulomatous reaction. In addition, they are in line with the reported declining role of interleukin-1 in the maintenance of granuloma.
对慢性肉芽肿性皮肤病（如皮肤利什曼病、环状肉芽肿、麻风病和汗腺炎）的多聚甲醛固定组织进行研究，以检测细胞浸润中白细胞介素 -1α、白细胞介素 -1β、白细胞介素 -6 和肿瘤坏死因子 -α 样免疫反应性的存在。在肉芽肿块周边和皮肤附属器周围，浆细胞对白细胞介素 -6 和肿瘤坏死因子 -α 有弱至强的细胞质标记。白细胞介素 -6 样免疫反应性似乎与细胞染色质物质的粗糙程度相关，染色质粗糙时更为强烈。浆细胞中白细胞介素 -1α 和白细胞介素 -1β 的细胞质标记较弱。上皮样细胞、朗汉斯巨细胞和小圆形细胞对白细胞介素 -1α 和白细胞介素 -1β 有弱至中度的细胞质标记，而白细胞介素 -6 和肿瘤坏死因子 -α 的染色强度为弱至强。此外，用抗白细胞介素 -1α、白细胞介素 -1β、白细胞介素 -6 和肿瘤坏死因子 -α 的抗血清对肉芽肿中心的基质进行染色。该区域细胞很少，表明肉芽肿处于消退过程。在所研究的皮肤疾病中这些细胞因子的出现提示白细胞介素 -6 和肿瘤坏死因子 -α 至少在维持期对肉芽肿反应有作用。这些发现也与 B 细胞在肉芽肿反应后期所起作用的推测一致。此外，它们与报道的白细胞介素 -1 在维持肉芽肿中的作用下降相符。
