Mitchell B W, King D J
U.S. Department of Agriculture, Southeast Poultry Research Laboratory, Athens, Georgia 30605.
Avian Dis. 1994 Oct-Dec;38(4):725-32.
Four-week-old mixed-sex White Rock chickens were used in four experiments to determine the effect of negative air ion enrichment on airborne transmission of the Roakin strain of Newcastle disease virus (NDV). The experiments were conducted in specially constructed airborne disease transmission cabinets in which donor (upwind) chickens cannot contact susceptible (downwind) chickens because of physical separation by a "no man's land." Temperature and humidity were computer-controlled at 26.7 C and 50% relative humidity, and ventilation rates were manually adjusted from 0.34 to 1.36 m3/min (12 to 48 ft3/min). Donor chickens were inoculated with Roakin NDV by eyedrop and intranasal routes and placed in the upwind end of each cabinet. One day later, susceptible chickens were placed in the downwind end. Seroconversion (> or = 1:10 NDV hemagglutination-inhibition titer) was considered evidence of infection from inoculation (upwind) or airborne transmission (downwind). Commercial air ion generators were used either in the ends or in the "no man's land" of the treatment cabinets and operated at power supply voltages ranging from -8kV direct current to -15 kV direct current. The use of negative air ion generators reduced airborne transmission an average of 6.6% to 27.7% compared with the control cabinets. Significant (P < or = 0.05) reductions in transmission were obtained with some treatments. The greatest reduction in transmission was obtained with the higher power supply voltages (13.8% reduction) and when the ionizers were placed in the "no man's land" (27.7% reduction) between the upwind and downwind chickens.
在四项实验中，使用了四周龄的混合性别的白洛克鸡，以确定空气负离子富集对新城疫病毒（NDV）罗阿金株空气传播的影响。实验在专门建造的空气传播疾病传输柜中进行，由于有“无人区”的物理隔离，供体（上风处）鸡无法接触易感（下风处）鸡。温度和湿度由计算机控制在26.7摄氏度和50%相对湿度，通风率手动调整为0.34至1.36立方米/分钟（12至48立方英尺/分钟）。通过滴眼和滴鼻途径给供体鸡接种罗阿金NDV，并将其放置在每个柜子的上风端。一天后，将易感鸡放置在下风端。血清转化（新城疫病毒血凝抑制效价≥1:10）被视为接种（上风处）或空气传播（下风处）感染的证据。在处理柜的两端或“无人区”使用商用空气离子发生器，其工作电源电压范围为-8kV直流至-15kV直流。与对照柜相比，使用空气负离子发生器使空气传播平均减少了6.6%至27.7%。一些处理获得了显著（P≤0.05）的传播减少。在较高电源电压下（减少13.8%）以及将离子发生器放置在上风鸡和下风鸡之间的“无人区”时（减少27.7%），传播减少最大。
