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人源和大肠杆菌切除核酸酶受乙酰氨基芴 - 鸟嘌呤加合物序列环境的影响不同。

Human and E.coli excinucleases are affected differently by the sequence context of acetylaminofluorene-guanine adduct.

作者信息

Mu D, Bertrand-Burggraf E, Huang J C, Fuchs R P, Sancar A, Fuchs B P

机构信息

Department of Biochemistry and Biophysics, University of North Carolina, School of Medicine, Chapel Hill.

出版信息

Nucleic Acids Res. 1994 Nov 25;22(23):4869-71. doi: 10.1093/nar/22.23.4869.

DOI:10.1093/nar/22.23.4869
PMID:7702657
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC523749/
Abstract

Synthetic DNA substrates containing an acetylaminofluorene (AAF) adduct at each of the three guanine in the G1G2CG3CC sequence were constructed and tested as substrates for reconstituted E.coli (A)BC excinuclease and human excinuclease in HeLa cell-free extract (CFE). The (A)BC excinulcease repaired the three substrates with relative efficiencies of G1:G2:G3 of 100:18:66 in agreement with an earlier report [Seeberg, E., and Fuchs, R.P.P. (1990) Proc. Natl Acad. Sci. USA 87, 191-194]. The same lesions were repaired by the human excinuclease with the strikingly different efficiencies of G1:G2:G3 as 38:100:68. These results reveal that the human excinuclease is affected by the sequence context of the lesion in a different manner than its prokaryotic counterpart.

摘要

构建了在G1G2CG3CC序列的三个鸟嘌呤位点上均含有乙酰氨基芴（AAF）加合物的合成DNA底物，并将其作为底物，用于在无细胞的HeLa细胞提取物（CFE）中重构的大肠杆菌（A）BC核酸外切酶和人核酸外切酶。（A）BC核酸外切酶修复这三种底物的相对效率为G1:G2:G3 = 100:18:66，这与之前的一份报告一致[西伯，E.，和富克斯，R.P.P.（1990年）《美国国家科学院院刊》87，191 - 194]。相同的损伤由人核酸外切酶修复，其G1:G2:G3的效率显著不同，为38:100:68。这些结果表明，人核酸外切酶受损伤序列背景的影响方式与其原核对应物不同。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d8d/523749/fbb4c8e69296/nar00047-0016-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d8d/523749/fbb4c8e69296/nar00047-0016-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0d8d/523749/fbb4c8e69296/nar00047-0016-a.jpg

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