Osaki T, Tatemoto Y, Yoneda K, Yamamoto T
Department of Oral Surgery, Kochi Medical School, Japan.
Eur J Cancer B Oral Oncol. 1994 Sep;30B(5):296-301. doi: 10.1016/0964-1955(94)90028-0.
We investigated the biological and histopathological characteristics of seven human tumour cell lines established from primary tongue squamous cell carcinoma (OSC-1), from metastasised lymph nodes of the gingival carcinoma (OSC-2 and OSC-3) and from tongue carcinoma (remaining four lines). The doubling time ranged from 22 h (OSC-2 and OSC-4) to 55 h (OSC-7), and did not correlate with tumour cell stratification in a collagen gel matrix. An invasive tendency was most prominent in OSC-2 and OSC-4; with the other cell lines, except OSC-6 and OSC-7, only a few sporadic invading cells were found in the tissue culture. In the cell lines established from the metastatised tumours, originally exhibiting grade 3 invasion, the invasion became more sporadic when the tumour cells were xenografted into the tongues of nude mice, while an invasion similar to the original was observed in the cell lines obtained from the original site (OSC-1) and from tumours of Grade 4C invasion. These findings suggest that the biological behaviour of the established tumour cells is markedly different even in tumours of the same tissue origin, and strongly invasive cells may selectively invade, and metastatise to the lymph nodes.
我们研究了七种人肿瘤细胞系的生物学和组织病理学特征，这些细胞系分别源自原发性舌鳞状细胞癌（OSC - 1）、牙龈癌转移淋巴结（OSC - 2和OSC - 3）以及舌癌（其余四种细胞系）。倍增时间从22小时（OSC - 2和OSC - 4）到55小时（OSC - 7）不等，且与肿瘤细胞在胶原凝胶基质中的分层无关。侵袭倾向在OSC - 2和OSC - 4中最为显著；对于其他细胞系，除了OSC - 6和OSC - 7，在组织培养中仅发现少数散在的侵袭细胞。在源自转移肿瘤（最初表现为3级侵袭）的细胞系中，当肿瘤细胞异种移植到裸鼠舌中时，侵袭变得更加散在，而在从原发部位（OSC - 1）获得的细胞系以及4C级侵袭肿瘤的细胞系中观察到与原来相似的侵袭情况。这些发现表明，即使在相同组织起源的肿瘤中，所建立的肿瘤细胞的生物学行为也明显不同，且强侵袭性细胞可能会选择性地侵袭并转移至淋巴结。
