Kawashiri S, Kumagai S, Kojima K, Harada H, Yamamoto E
Department of Oral and Maxillofacial Surgery, School of Medicine, Kanazawa University, Japan.
Eur J Cancer B Oral Oncol. 1995 Jul;31B(4):216-21. doi: 10.1016/0964-1955(95)00027-f.
A new model was devised in order to establish an in vivo model for oral carcinoma that exhibits significant local invasion and metastasis. One hundred and fifty-two nude mice had tumour cells from one of two established oral squamous cell carcinoma (SCC) cell lines (OSC-19 and OSC-20) implanted into the tongue or the oral floor via an intra-oral route and, as a control, the subcutaneous tissue of the back. The back tumours showed an expansive growth pattern, lacking significant invasion of surrounding tissues. In contrast, the tumours implanted into the tongue or the oral floor exhibited invasive growth and the histological appearance was similar to that of the original tumours. Moreover, regional neck lymph node and pulmonary metastases were observed in this model. Regional neck lymph node metastases were detected in 81.0% of mice implanted with OSC-19 cells and in 13.6% of mice implanted with OSC-20 cells. OSC-19 and OSC-20 cells showed pulmonary metastases in 9.5 and 9.1% of mice, respectively. These results suggest that this intra-oral implantation model is valuable in the study of the mechanism of invasion and metastasis of oral SCC.
为建立一种能显著表现出局部侵袭和转移的口腔癌体内模型，设计了一种新模型。152只裸鼠通过口腔内途径将来自两种已建立的口腔鳞状细胞癌（SCC）细胞系（OSC-19和OSC-20）之一的肿瘤细胞植入舌部或口腔底部，作为对照，将肿瘤细胞植入背部皮下组织。背部肿瘤呈现膨胀性生长模式，对周围组织无明显侵袭。相比之下，植入舌部或口腔底部的肿瘤表现为侵袭性生长，组织学外观与原发肿瘤相似。此外，在该模型中观察到区域颈部淋巴结和肺转移。植入OSC-19细胞的小鼠中有81.0%检测到区域颈部淋巴结转移，植入OSC-20细胞的小鼠中有13.6%检测到区域颈部淋巴结转移。OSC-19和OSC-20细胞分别在9.5%和9.1%的小鼠中出现肺转移。这些结果表明，这种口腔内植入模型在研究口腔SCC侵袭和转移机制方面具有重要价值。
