Wu H C, Lin J J
J Bacteriol. 1976 Apr;126(1):147-56. doi: 10.1128/jb.126.1.147-156.1976.
Mutants with alterations in the structure, biosynthesis, or assembly of murein lipoprotein were selected by a procedure based on radiation suicide of wild-type organisms by [3H]arginine under conditions where the radioactive arginine was preferentially incorporated into lipoprotein. Further screening for the potential mutants among the survivors of [3H]arginine suicide was carried out by using a sensitive immunodiffusion test, followed by radioactive double-labeling experiments. Three mutants were obtained and partially characterized.
通过一种程序筛选出了在胞壁质脂蛋白的结构、生物合成或组装方面发生改变的突变体。该程序基于在放射性精氨酸优先掺入脂蛋白的条件下,利用[3H]精氨酸对野生型生物体进行辐射自杀。通过使用灵敏的免疫扩散试验,随后进行放射性双标记实验,对[3H]精氨酸自杀幸存者中的潜在突变体进行了进一步筛选。获得了三个突变体并对其进行了部分特性分析。