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在胞壁质脂蛋白方面发生改变的大肠杆菌突变体。

Escherichia coli mutants altered in murein lipoprotein.

作者信息

Wu H C, Lin J J

出版信息

J Bacteriol. 1976 Apr;126(1):147-56. doi: 10.1128/jb.126.1.147-156.1976.

Abstract

Mutants with alterations in the structure, biosynthesis, or assembly of murein lipoprotein were selected by a procedure based on radiation suicide of wild-type organisms by [3H]arginine under conditions where the radioactive arginine was preferentially incorporated into lipoprotein. Further screening for the potential mutants among the survivors of [3H]arginine suicide was carried out by using a sensitive immunodiffusion test, followed by radioactive double-labeling experiments. Three mutants were obtained and partially characterized.

摘要

通过一种程序筛选出了在胞壁质脂蛋白的结构、生物合成或组装方面发生改变的突变体。该程序基于在放射性精氨酸优先掺入脂蛋白的条件下,利用[3H]精氨酸对野生型生物体进行辐射自杀。通过使用灵敏的免疫扩散试验,随后进行放射性双标记实验,对[3H]精氨酸自杀幸存者中的潜在突变体进行了进一步筛选。获得了三个突变体并对其进行了部分特性分析。

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More mutant tyrosine transfer ribonucleic acids.更多突变型酪氨酸转移核糖核酸。
J Mol Biol. 1970 Nov 28;54(1):1-14. doi: 10.1016/0022-2836(70)90442-0.
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A mutation which changes a membrane protein of E. coli.一种改变大肠杆菌膜蛋白的突变。
Proc Natl Acad Sci U S A. 1969 Nov;64(3):957-61. doi: 10.1073/pnas.64.3.957.

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