Chattopadhyay P K, Engel R, Tropp B E, Wu H C
J Bacteriol. 1979 Jun;138(3):944-8. doi: 10.1128/jb.138.3.944-948.1979.
The effects of 3,4-dihydroxybutyl-1-phosphonate, a four-carbon analog of sn-glycerol 3-phosphate, on the biosynthesis of the glyceryl moiety in murein lipoprotein of Escherichia coli were studied. The compound at a concentration of 55 microM strong inhibits in the incorporation of [2-3H]glycerol radioactivity into lipoprotein by virtue of its inhibition of the synthesis of phosphatidylglycerol. On the other hand, the incorporation of prelabeled [2-3H]glycerol radioactivity into lipoprotein was only partially inhbited by 3,4-dihydroxybutyl-1-phosphonate even at a much higher concentration (1 mM). These data were consistent with the postulated pathway for the biosynthesis of the lipid moiety in lipoportein: cysteine-lipoprotein + phosphatidylglycerol leads to glycerylcystein-lipoprotein + phosphatidic acid.
研究了3,4-二羟基丁基-1-膦酸酯（一种sn-甘油3-磷酸的四碳类似物）对大肠杆菌胞壁质脂蛋白中甘油部分生物合成的影响。该化合物浓度为55微摩尔时，由于其对磷脂酰甘油合成的抑制作用，强烈抑制了[2-³H]甘油放射性掺入脂蛋白。另一方面，即使在高得多的浓度（1毫摩尔）下，3,4-二羟基丁基-1-膦酸酯也只是部分抑制预先标记的[2-³H]甘油放射性掺入脂蛋白。这些数据与脂蛋白中脂质部分生物合成的假定途径一致：半胱氨酸-脂蛋白 + 磷脂酰甘油 → 甘油半胱氨酸-脂蛋白 + 磷脂酸。
