Reenilä I, Tuomainen P, Männistö P T
Department of Pharmacology and Toxicology, University of Helsinki, Finland.
J Chromatogr B Biomed Appl. 1995 Jan 6;663(1):137-42. doi: 10.1016/0378-4347(94)00433-6.
We applied coulometric detection (three electrochemical electrodes in series) to quantitate vanillic acid and isovanillic acid using reversed-phase HPLC. The formation of these reaction products from dihydroxybenzoic acid was used as a precise and reproducible measure of catechol-O-methyltransferase (COMT) activity in striatal homogenates and recombinant membrane-bound COMT protein. This detection system has a higher sensitivity (0.5 pmol per injection) than a single-cell amperometric detection. As in a previous method, the deproteinized supernatants of the COMT assay could be injected directly onto the HPLC system allowing the handling of a large number of samples in one day.
我们应用库仑检测法（三个电化学电极串联），通过反相高效液相色谱法对香草酸和异香草酸进行定量。二羟基苯甲酸生成这些反应产物的过程被用作纹状体匀浆和重组膜结合儿茶酚-O-甲基转移酶（COMT）蛋白中COMT活性的精确且可重复的测量方法。该检测系统比单细胞安培检测具有更高的灵敏度（每次进样0.5皮摩尔）。与之前的方法一样，COMT测定的脱蛋白上清液可直接注入高效液相色谱系统，从而能够在一天内处理大量样品。
