van Dijk J, Boomsma F, Alberts G, Man in 't Veld A J, Schalekamp M A
Division of Internal Medicine I, University Hospital Dijkzigt/Erasmus University, Rotterdam, Netherlands.
J Chromatogr B Biomed Appl. 1995 Jan 6;663(1):43-50. doi: 10.1016/0378-4347(94)00408-w.
We report here a simple and sensitive method for the measurement of semicarbazide-sensitive amine oxidase (SSAO) activity in human plasma. Benzaldehyde, generated during a 1-h incubation of plasma with benzylamine, is derivatized with the specific aldehyde reagent dimedone after prior deproteinization. Quantitation of the derivatization product is done by automated injection onto an isocratic high-performance liquid chromatographic system with fluorimetric detection. The assay shows good linearity and reproducibility (intra-assay C.V. 7%). Detection limit is 25 mU/l (= pmol/ml/min). In 51 healthy controls (age 49 +/- 13 yr, 20 males) the measured SSAO activity was 352 +/- 102 mU/l (mean +/- S.D.). A large number of samples (70-80) can easily be processed in one day by one technician.
我们在此报告一种用于测量人血浆中氨基脲敏感性胺氧化酶（SSAO）活性的简单且灵敏的方法。在用苄胺将血浆孵育1小时的过程中产生的苯甲醛，在预先进行脱蛋白处理后，用特定的醛试剂达美酮进行衍生化。通过自动进样到配备荧光检测的等度高效液相色谱系统上对衍生化产物进行定量。该测定法显示出良好的线性和重现性（批内变异系数7%）。检测限为25 mU/l（= pmol/ml/分钟）。在51名健康对照者（年龄49±13岁，20名男性）中，测得的SSAO活性为352±102 mU/l（平均值±标准差）。一名技术人员一天内可以轻松处理大量样本（70 - 80个）。
