Foglino M, Borne F, Bally M, Ball G, Patte J C
Laboratoire de Chimie Bactérienne, Centre National de la Recherche Scientifique, Marseille, France.
Microbiology (Reading). 1995 Feb;141 ( Pt 2):431-9. doi: 10.1099/13500872-141-2-431.
The relationship between genes and enzymes in the methionine biosynthetic pathway has been studied in Pseudomonas aeruginosa. The first step is catalysed by an O-succinylhomoserine synthase, the product of the metA gene mapped at 20 min on the chromosome. The second step is achieved by direct sulfhydrylation, involving the enzyme encoded by a metZ gene that we have identified and sequenced, located at 40 min. Thus Pseudomonas appears to be the only organism so far described that uses O-succinylhomoserine as substrate for a direct sulfhydrylation. As in yeast, the two transsulfuration pathways between cysteine and homocysteine, with cystathionine as an intermediate, probably exist in parallel in this organism.
人们已经在铜绿假单胞菌中研究了甲硫氨酸生物合成途径中基因与酶之间的关系。第一步由O-琥珀酰高丝氨酸合酶催化，该酶是位于染色体上20分钟处的metA基因的产物。第二步通过直接巯基化实现，涉及我们已鉴定和测序的位于40分钟处的metZ基因所编码的酶。因此，假单胞菌似乎是迄今为止所描述的唯一一种使用O-琥珀酰高丝氨酸作为直接巯基化底物的生物体。与酵母一样，在该生物体中可能也同时存在以胱硫醚为中间体的半胱氨酸和高半胱氨酸之间的两条转硫途径。
