Davis R E, Kelly T J, Masler E P, Thyagaraja B S, Rote C A, Imberski R B
Insect Neurobiology and Hormone Laboratory, USDA, Beltsville, Maryland 20705.
Insect Mol Biol. 1994 Nov;3(4):219-28. doi: 10.1111/j.1365-2583.1994.tb00170.x.
A 1355 bp sequence (accession number L32141) isolated from a gypsy moth (Lymantria dispar) cDNA library showed 68-74% sequence identity to mitochondrial large subunit ribosomal RNA (mt IrRNA) sequences of Locusta migratoria, Apis mellifera, Aedes albopictus, Anopheles gambiae and two Drosophila species. A comparison of the primary sequences of the mt IrRNAs from the above insects in four orders and from Esherichia coli demonstrated regions of conservation which presumably correspond to regions of functional and/or structural homology. A secondary structure for the gypsy moth mt IrRNA sequence was derived based on the proposed secondary structures of Drosophila yakuba and Aedes albopictus mt IrRNAs (Gutell & Fox, Nucleic Acid Res 16 (Suppl.), r175-r269, 1988). This sequence was found to hybridize to about 10-15% of the clones in several (eleven) gypsy moth cDNA libraries.
从舞毒蛾（Lymantria dispar）cDNA文库中分离出的一段1355 bp序列（登录号L32141）与飞蝗（Locusta migratoria）、意大利蜜蜂（Apis mellifera）、白纹伊蚊（Aedes albopictus）、冈比亚按蚊（Anopheles gambiae）以及两种果蝇的线粒体大亚基核糖体RNA（mt IrRNA）序列具有68 - 74%的序列同一性。对上述四个目昆虫以及大肠杆菌的mt IrRNAs一级序列进行比较，发现了一些保守区域，这些区域可能对应于功能和/或结构同源区域。基于雅库布果蝇（Drosophila yakuba）和白纹伊蚊mt IrRNAs的二级结构模型（Gutell & Fox，《核酸研究》16（增刊），r175 - r269，1988），推导了舞毒蛾mt IrRNA序列的二级结构。在几个（11个）舞毒蛾cDNA文库中，发现该序列与约10 - 15%的克隆发生杂交。
