Nolte M, Werner M, Spann W, Schnabel B, von Wasielewski R, Wilkens L, Hübner K, Fischer R, Georgii A
Pathologisches Institut, Medizinischen Hochschule Hannover, Germany.
Virchows Arch. 1995;426(1):37-41. doi: 10.1007/BF00194696.
Ninety-one Hodgkin's lymphomas (HD), 52 non-Hodgkin lymphomas (NHL) and 33 specimens of non-neoplastic lymphatic tissues were investigated by polymerase chain reaction (PCR) for the presence of the bcl-2/JH gene rearrangement. The majority of the HD cases were drawn from the files of the German Hodgkin trial where diagnoses are established by a panel of four independent histopathologists. Using the very sensitive PCR method which detected 1 positive among 10000 negative cells, the bcl-2/JH gene rearrangement was found in 7/52 NHL and 3/16 tonsils with follicular hyperplasia, but in none of the 91 HD. The bcl-2 protein, however, was expressed by malignant cells of B and T cell lymphomas and by the giant tumour cells in 2/13 HD lymphocyte predominant, 11/28 HD nodular sclerosing I, 14/17 HD nodular sclerosing II, 10/27 HD mixed cellularity and 3/3 HD lymphocyte depleted. The bcl-2/JH rearrangement is thus independent of protein over-expression, the latter being found in all types of lymphomas. Our results do not confirm the findings of others who have detected the bcl-2/JH rearrangement in HD. These discrepancies may be explained by differences in choice of material, the gene rearrangement actually occurring in bystander cells but not in Reed-Sternberg or Hodgkin cells, or by contamination.
采用聚合酶链反应（PCR）对91例霍奇金淋巴瘤（HD）、52例非霍奇金淋巴瘤（NHL）以及33例非肿瘤性淋巴组织标本进行bcl-2/JH基因重排检测。多数HD病例来自德国霍奇金试验的病例档案，其诊断由四位独立的组织病理学家组成的小组确定。使用极为灵敏的PCR方法（可在10000个阴性细胞中检测出1个阳性细胞），在52例NHL中的7例以及16例伴有滤泡增生的扁桃体中的3例检测到bcl-2/JH基因重排，但在91例HD中均未检测到。然而，bcl-2蛋白在B和T细胞淋巴瘤的恶性细胞以及2/13例淋巴细胞为主型HD、11/28例结节硬化型I HD、14/17例结节硬化型II HD、10/27例混合细胞型HD和3/3例淋巴细胞消减型HD的巨肿瘤细胞中表达。因此，bcl-2/JH重排与蛋白过表达无关，后者在所有类型的淋巴瘤中均有发现。我们的结果未证实其他人在HD中检测到bcl-2/JH重排的研究结果。这些差异可能是由于材料选择的不同、基因重排实际上发生在旁观者细胞而非里德-斯腾伯格细胞或霍奇金细胞中，或者是由于污染所致。
