Alkan S, Ross C W, Hanson C A, Schnitzer B
Department of Pathology, University of Michigan, Ann Arbor, USA.
Mod Pathol. 1995 Jun;8(5):544-7.
Recent studies have indicated that nodular lymphocyte predominance Hodgkin's disease (nLP-HD) is a B-cell lymphoma. Although molecular events in the neoplastic transformation of B-cells are not well understood, Epstein-Barr virus infection and bcl-2 protein overexpression have been postulated to have etiologic roles in some lymphomas. Epstein-Barr virus has been demonstrated in the Reed-Sternberg cells of Hodgkin's disease cases (other than nLP-HD) as well as in some B-cell lymphomas; bcl-2 overexpression is found in the majority of follicular lymphomas. The biologic role for bcl-2 in HD is controversial. Some reports have indicated the presence of bcl-2 gene rearrangements, associated with the t(14;18) (q32;q21), detected by the polymerase chain reaction in HD; recent studies have failed to confirm this finding. Reports in the literature describe only a few such analyses in the nLP-HD subtype. To address these conflicting issues, we examined 12 cases of nLP-HD to determine whether bcl-2 protein expression (by immunohistochemistry) and Epstein-Barr virus (by in situ hybridization) could be detected in the Reed-Sternberg variants. None of the cases showed expression of bcl-2 protein or Epstein-Barr virus RNA in the neoplastic cells. Epstein-Barr virus does not appear to play an important role in the pathogenesis of nLP-HD. Similarly, we cannot substantiate a role for bcl-2 in the development of this type of Hodgkin's disease.
近期研究表明，结节性淋巴细胞为主型霍奇金淋巴瘤（nLP-HD）是一种B细胞淋巴瘤。尽管B细胞肿瘤转化中的分子事件尚未完全明确，但据推测，爱泼斯坦-巴尔病毒感染和bcl-2蛋白过表达在某些淋巴瘤的病因学中具有一定作用。在霍奇金淋巴瘤病例（nLP-HD除外）的里德-斯腾伯格细胞以及一些B细胞淋巴瘤中均已证实存在爱泼斯坦-巴尔病毒；在大多数滤泡性淋巴瘤中发现有bcl-2过表达。bcl-2在霍奇金淋巴瘤中的生物学作用存在争议。一些报告指出，通过聚合酶链反应在霍奇金淋巴瘤中检测到与t(14;18)（q32;q21）相关的bcl-2基因重排；但近期研究未能证实这一发现。文献报道中仅对nLP-HD亚型进行了少数此类分析。为解决这些相互矛盾的问题，我们检测了12例nLP-HD病例，以确定在里德-斯腾伯格变异细胞中是否能检测到bcl-2蛋白表达（通过免疫组织化学）和爱泼斯坦-巴尔病毒（通过原位杂交）。所有病例的肿瘤细胞中均未显示bcl-2蛋白表达或爱泼斯坦-巴尔病毒RNA。爱泼斯坦-巴尔病毒似乎在nLP-HD的发病机制中不起重要作用。同样，我们也无法证实bcl-2在这类霍奇金淋巴瘤发生过程中的作用。
