Willhoeft U, Traut W
Institut für Biologie, Medizinische Universität zu Lübeck, Germany.
Chromosome Res. 1995 Jan;3(1):59-65. doi: 10.1007/BF00711163.
In Megaselia scalaris (Loew) the presence or absence of a male-determining factor, M, is responsible for sex determination. In two wild-type strains, M is located on the homomorphic chromosome pair 2. In the laboratory line Except42 a new Y chromosome was created by recombination between the original Y and the original X chromosome. The Except42 Y chromosome has conserved the sex-determining function and four molecular markers of the original Y chromosome, while 13 original Y markers have been lost. The new Y chromosome, therefore, consists of roughly one-quarter of the original Y chromosome and three-quarters of the original X chromosome. To define the sex-determining region, cosmid clones, one from the original X and one from the original Y chromosome region of the Except42 Y chromosome, were isolated and used as probes for chromosomal in situ suppression (CISS) hybridization. The CISS hybridization signals map the conserved Y segment, including the male-determining factor, to the distal segment of the short arm of the Y chromosome.
在黑腹果蝇（Megaselia scalaris，Loew）中，雄性决定因子M的存在与否决定了性别。在两个野生型品系中，M位于同态的第2号染色体对。在实验室品系Except42中，通过原始Y染色体与原始X染色体之间的重组产生了一条新的Y染色体。Except42 Y染色体保留了性别决定功能以及原始Y染色体的四个分子标记，而13个原始Y染色体标记已经丢失。因此，新的Y染色体大约由原始Y染色体的四分之一和原始X染色体的四分之三组成。为了确定性别决定区域，从Except42 Y染色体的原始X染色体区域和原始Y染色体区域分离出黏粒克隆，并将其用作染色体原位抑制（CISS）杂交的探针。CISS杂交信号将包括雄性决定因子在内的保守Y片段定位到Y染色体短臂的远端片段。
