Deacon T W, Pakzaban P, Isacson O
Neuroregeneration Laboratory, McLean Hospital, Belmont, MA 02178, USA.
Brain Res. 1994 Dec 30;668(1-2):211-9. doi: 10.1016/0006-8993(94)90526-6.
In order to determine whether the lateral ganglionic eminence (LGE) of the fetal telencephalon is the primary source of striatal precursors in striatal transplants and tissue cultures, cells derived exclusively from the LGE of fetal rat brains were transplanted into the quinolinic-acid-lesioned striatum of adult rats. After 2-3 months they produced grafts that were almost entirely AChE-positive as well as DARPP-32-, TH-, and calbindin-immunoreactive. The grafts were integrated into the host striatum so that host corticofugal fiber tracts interdigitated with graft tissues similar to the way they penetrate the gray matter of the normal striatum. Fast Blue dye injected into the ipsilateral globus pallidus of LGE grafted produced retrogradely labeled neurons within the grafts, but Fluorogold dye injected into the ipsilateral substantia nigra did not. In a separate experiment using DARPP-32-immunohistochemstry as a striatal marker, fetal (E16) and neonatal (P2) rat brains showed DARPP-32 immunoreactivity in the LGE but not in the adjacent medial ganglionic eminence (MGE). In summary, both fetal LGE cells and LGE grafts express specific striatal markers, and LGE grafts integrate into the host striatum and innervate the major striatal efferent target within the host brain. These data suggest that the LGE is the origin of cells committed to striatal phenotypes in the developing brain.
为了确定胎儿端脑的外侧神经节隆起（LGE）是否是纹状体移植和组织培养中纹状体前体的主要来源，将仅来源于胎鼠脑LGE的细胞移植到成年大鼠经喹啉酸损伤的纹状体中。2至3个月后，它们产生的移植物几乎完全呈乙酰胆碱酯酶（AChE）阳性，以及对多巴胺和腺苷酸环化酶相关磷酸蛋白-32（DARPP-32）、酪氨酸羟化酶（TH）和钙结合蛋白呈免疫反应性。移植物整合到宿主纹状体中，使得宿主皮质离心纤维束与移植物组织相互交错，类似于它们穿透正常纹状体灰质的方式。向移植了LGE的同侧苍白球注射快蓝染料，在移植物中产生了逆行标记的神经元，但向同侧黑质注射荧光金染料则没有。在另一个使用DARPP-32免疫组织化学作为纹状体标记物的实验中，胎鼠（E16）和新生鼠（P2）的脑在LGE中显示出DARPP-32免疫反应性，但在相邻的内侧神经节隆起（MGE）中未显示。总之，胎儿LGE细胞和LGE移植物均表达特定的纹状体标记物，并且LGE移植物整合到宿主纹状体中并支配宿主脑内主要的纹状体传出靶标。这些数据表明，LGE是发育中脑内致力于形成纹状体表型的细胞的起源。
