Martínez-Palomo A, DeSouza W, Gonzalez-Robles A
J Cell Biol. 1976 May;69(2):507-13. doi: 10.1083/jcb.69.2.507.
A regional specialization of the cell surface of T. cruzi culture forms was found at the cytostome as a localized thick surface coat rich in carbohydrate-containing components. The prominent surface coat was located over a region of the plasma membrane where intramembranous particles were exceedingly low in number. In turn, the particle-poor region was related to specialized submembrane fibrils not present under other regions of the plasma membrane. The cystostome region provides a striking example of a stable regional differentiation of the plasma membrane, involving the outer surface, the membrane interior, and the underlying cytoplasm. In addition, independence of Con A receptors, colloidal iron binding sites, and ruthenium red-stainable surface components from membrane particles was demonstrated at the flagellar membrane.
在克氏锥虫培养形式的细胞表面，发现胞口处存在区域特化现象，表现为富含含碳水化合物成分的局部厚表面层。显著的表面层位于质膜的一个区域上，该区域的膜内颗粒数量极少。反过来，颗粒贫乏区域与质膜其他区域下方不存在的特化膜下纤维有关。胞口区域提供了一个质膜稳定区域分化的显著例子，涉及外表面、膜内部和下方的细胞质。此外，在鞭毛膜处证明了刀豆球蛋白A受体、胶体铁结合位点和钌红可染色表面成分与膜颗粒无关。
