Shahabuddin S
Department of Pathology (32), College of Medicine, King Saud University, Riyadh, Saudi Arabia.
Clin Immunol Immunopathol. 1995 May;75(2):168-70. doi: 10.1006/clin.1995.1067.
In an attempt to establish the reference ranges for lymphocyte subsets the distribution of lymphocyte-population-bearing surface markers such as CD3 (T cells), CD19 (B cells), CD4 (T helper/inducer cells), CD8 (T suppressor/cytotoxic cells), HLA-DR on CD3+ cells (activated T cells), and CD16 and/or CD56 on CD3- cells (NK cells) has been studied among normal male Saudi blood donors. Anticoagulated peripheral blood was stained with monoclonal antibodies and the lymphocytes were analyzed by flow cytometry for the expression of the above markers. Reference ranges were as follows: CD3+ (61 to 87%), CD19+ (5 to 27%), CD4+ (31 to 55%), CD8+ (24 to 56%), CD4+/CD8+ ratio (0.5 to 1.8), CD3+ HLA-DR+ (4 to 29%), and CD3- CD16+/CD56+ (4 to 29%). Furthermore, the absolute cell numbers for each lymphocyte subset are reported here for the first time for the Saudi male population. The most significant finding of the present study is the presence of a higher percentage and number of CD8+ T cells (P < 0.01) and a decreased CD4/CD8 ratio (P < 0.02) compared with the Caucasian controls. In addition, the Saudi male population has a significantly lower percentage and number of activated T cells (P < 0.05 and < 0.01, respectively) and a lower number of NK cells (P < 0.001).
为了确定淋巴细胞亚群的参考范围，我们研究了正常沙特男性献血者中携带表面标志物的淋巴细胞群体的分布情况，这些标志物包括CD3（T细胞）、CD19（B细胞）、CD4（T辅助/诱导细胞）、CD8（T抑制/细胞毒性细胞）、CD3 +细胞上的HLA - DR（活化T细胞）以及CD3 -细胞上的CD16和/或CD56（NK细胞）。用单克隆抗体对抗凝外周血进行染色，并通过流式细胞术分析淋巴细胞上述标志物的表达情况。参考范围如下：CD3 +（61%至87%）、CD19 +（5%至27%）、CD4 +（31%至55%）、CD8 +（24%至56%）、CD4 + / CD8 +比值（0.5至1.8）、CD3 + HLA - DR +（4%至29%）以及CD3 - CD16 + / CD56 +（4%至29%）。此外，本文首次报道了沙特男性人群中各淋巴细胞亚群的绝对细胞数。本研究最显著的发现是，与白种人对照组相比，CD8 + T细胞的百分比和数量更高（P < 0.01），CD4 / CD8比值降低（P < 0.02）。此外，沙特男性人群中活化T细胞的百分比和数量显著更低（分别为P < 0.05和< 0.01），NK细胞数量也更低（P < 0.001）。
