Croco J L, Erwin M E, Jennings J M, Putnam L R, Jones R N
Department of Pathology, University of Iowa College of Medicine, Iowa City 52242.
Diagn Microbiol Infect Dis. 1994 Dec;20(4):213-9. doi: 10.1016/0732-8893(94)90006-x.
One hundred ninety-seven anaerobic organisms (24 Gardnerella vaginalis, 16 Mobiluncus spp., 19 Peptostreptococcus spp., 20 Lactobacillus spp., 20 Prevotella bivia/disiens, 81 Bacteroides fragilis group, 12 Clostridium spp., and five Fusobacterium spp.) were processed by the Etest (AB Biodisk, Solna, Sweden) and a reference (Brucella blood agar) method against 10 antimicrobial agents. For the bacterial vaginosis-associated pathogens, the Etest was more reproducible and correlated acceptably with the reference agar test: within +/- 1 log2 dilution for 74.4% of Mobiluncus spp. to 96.0% for Peptostreptococcus spp. (all organisms, 83.4%). The quantitative correlation +/- 2 log2 dilution steps between test results was 94.3%. Results with B. fragilis group strains demonstrated 97.3% correlation (+/- 2 log2 dilution) with a trend toward slightly lower Etest minimum inhibitory concentrations for ampicillin-sulbactam, cefotaxime, imipenem, and clindamycin. The absolute qualitative interpretive agreement between Etest and the reference agar dilution method results was 94.4%, with only a 0.4% false-susceptible error rate. The Etest appears to be a very practical, quantitatively accurate, alternative procedure for clinical microbiology laboratories routinely testing the susceptibilities of anaerobes and, by these presented data, organisms associated with female tract infections.
采用Etest法（AB生物盘公司，瑞典索尔纳）和参考方法（布鲁氏菌血琼脂），针对10种抗菌药物对197株厌氧菌（24株阴道加德纳菌、16株动弯杆菌属、19株消化链球菌属、20株乳杆菌属、20株二路普雷沃菌/双路普雷沃菌、81株脆弱拟杆菌群、12株梭菌属和5株具核梭杆菌）进行了检测。对于与细菌性阴道病相关的病原体，Etest法的重复性更好，与参考琼脂试验的相关性也可接受：动弯杆菌属的74.4%至消化链球菌属的96.0%（所有菌株为83.4%）在±1个log2稀释度范围内。检测结果之间±2个log2稀释度步骤的定量相关性为94.3%。脆弱拟杆菌群菌株的结果显示相关性为97.3%（±2个log2稀释度），对于氨苄西林 - 舒巴坦、头孢噻肟、亚胺培南和克林霉素，Etest法的最低抑菌浓度有略低的趋势。Etest法与参考琼脂稀释法结果的绝对定性解释一致性为94.4%，假敏感错误率仅为0.4%。对于临床微生物实验室常规检测厌氧菌以及根据这些数据检测与女性生殖道感染相关的病原体，Etest法似乎是一种非常实用、定量准确的替代方法。
