Felten A, Barreau C, Bizet C, Lagrange P H, Philippon A
Service de Bactériologie-Virologie-Hygiène, Hôpital St-Louis, 75475 Paris Cedex 10, France.
J Clin Microbiol. 1999 Mar;37(3):729-33. doi: 10.1128/JCM.37.3.729-733.1999.
Lactobacilli recovered from the blood, cerebrospinal fluid, respiratory tract, and gut of 20 hospitalized immunocompromised septic patients were analyzed. Biochemical carbohydrate fermentation and total soluble cell protein profiles were used to identify the species. Hydrogen peroxide production was measured. Susceptibility to 19 antibiotics was tested by a diffusion method, and the MICs of benzylpenicillin, amoxicillin, imipenem, erythromycin, vancomycin, gentamicin, and levofloxacin were determined. A small number of species produced H2O2, and antibiotic susceptibilities were species related. Eighteen (90%) of the isolates were L. rhamnosus, one was L. paracasei subsp. paracasei, and one was L. crispatus. L. rhamnosus, L. paracasei subsp. paracasei isolates, and the type strains were neither H2O2 producers nor vancomycin susceptible (MICs, >/=256 microgram/ml). L. crispatus, as well as most of the type strains of lactobacilli which belong to the L. acidophilus group, was an H2O2 producer and vancomycin susceptible (MICs, <4 microgram/ml).
对从20名住院免疫功能低下的败血症患者的血液、脑脊液、呼吸道和肠道中分离出的乳酸杆菌进行了分析。采用生化碳水化合物发酵和总可溶性细胞蛋白谱来鉴定菌种。测定了过氧化氢的产生量。通过扩散法检测了对19种抗生素的敏感性，并测定了苄青霉素、阿莫西林、亚胺培南、红霉素、万古霉素、庆大霉素和左氧氟沙星的最低抑菌浓度（MIC）。少数菌种产生过氧化氢，抗生素敏感性与菌种有关。18株（90%）分离株为鼠李糖乳杆菌，1株为副干酪乳杆菌副干酪亚种，1株为卷曲乳杆菌。鼠李糖乳杆菌、副干酪乳杆菌副干酪亚种分离株以及模式菌株既不产生过氧化氢，也对万古霉素不敏感（MICs，≥256微克/毫升）。卷曲乳杆菌以及嗜酸乳杆菌群中的大多数乳酸杆菌模式菌株是过氧化氢产生菌，且对万古霉素敏感（MICs，<4微克/毫升）。
