Charles A C
UCLA Department of Neurology 90024.
Dev Neurosci. 1994;16(3-4):196-206. doi: 10.1159/000112107.
There is increasing evidence for bidirectional communication between glial cells and neurons. In this study, calcium signaling in primary glia/neuron cultures was investigated using video fluorescence imaging and fura-2. Glial cells in culture without neurons showed occasional spontaneous intracellular Ca2+ oscillations but not intercellular Ca2+ waves. By contrast, glial cells in culture with neurons showed frequent spontaneous Ca2+ oscillations as well as propagated intercellular Ca2+ waves. These spontaneous glial intercellular Ca2+ waves often emanated from sites of contact with neurons, but were only occasionally associated with increases in neuronal Ca2+. Mechanical stimulation of a single glial cell induced a glial intercellular Ca2+ wave which was similar in its temporal and spatial characteristics to spontaneous glial Ca2+ waves. Mechanically induced glial Ca2+ waves, but not spontaneous Ca2+ waves, evoked a transient increase in [Ca2+]i or a change in the pattern of spontaneous Ca2+ oscillations in a small percentage (< 10%) of neighboring neurons. Mechanical stimulation of a single neuron consistently evoked an intercellular Ca2+ wave in neighboring glial cells. These results suggest distinct mechanisms for direct glial-neuronal and neuronal-glial communication. These signaling pathways may play important roles in both function and pathology in the central nervous system.
越来越多的证据表明神经胶质细胞和神经元之间存在双向通讯。在本研究中，使用视频荧光成像和fura-2对原代神经胶质细胞/神经元共培养物中的钙信号进行了研究。没有神经元的培养物中的神经胶质细胞偶尔会出现自发的细胞内Ca2+振荡，但没有细胞间Ca2+波。相比之下，与神经元共培养的神经胶质细胞则频繁出现自发的Ca2+振荡以及传播的细胞间Ca2+波。这些自发的神经胶质细胞间Ca2+波通常起源于与神经元接触的部位，但仅偶尔与神经元Ca2+的增加有关。对单个神经胶质细胞的机械刺激会诱导出一种神经胶质细胞间Ca2+波，其时间和空间特征与自发的神经胶质细胞Ca2+波相似。机械诱导的神经胶质细胞Ca2+波而非自发的Ca2+波，会在一小部分（<10%）相邻神经元中引起[Ca2+]i的短暂增加或自发Ca2+振荡模式的改变。对单个神经元的机械刺激始终会在相邻的神经胶质细胞中诱发细胞间Ca2+波。这些结果表明了神经胶质细胞 - 神经元和神经元 - 神经胶质细胞直接通讯的不同机制。这些信号通路可能在中枢神经系统的功能和病理学中都发挥重要作用。
