Charles A C, Merrill J E, Dirksen E R, Sanderson M J
Department of Neurology, UCLA School of Medicine 90024.
Neuron. 1991 Jun;6(6):983-92. doi: 10.1016/0896-6273(91)90238-u.
Intercellular Ca2+ signaling in primary cultures of glial cells was investigated with digital fluorescence video imaging. Mechanical stimulation of a single cell induced a wave of increased [Ca2+]i that was communicated to surrounding cells. This was followed by asynchronous Ca2+ oscillations in some cells. Similar communicated Ca2+ responses occurred in the absence of extracellular Ca2+, despite an initial decrease in [Ca2+]i in the stimulated cell. Mechanical stimulation in the presence of glutamate induced a typical communicated Ca2+ wave through cells undergoing asynchronous Ca2+ oscillations in response to glutamate. The coexistence of communicated Ca2+ waves and asynchronous Ca2+ oscillations suggests distinct mechanisms for intra- and intercellular Ca2+ signaling. This intercellular signaling may coordinate cooperative glial function.
利用数字荧光视频成像技术研究了神经胶质细胞原代培养物中的细胞间Ca2+信号传导。对单个细胞的机械刺激诱导了[Ca2+]i升高的波，该波传递至周围细胞。随后一些细胞出现异步Ca2+振荡。尽管受刺激细胞中的[Ca2+]i最初有所下降，但在没有细胞外Ca2+的情况下仍会出现类似的Ca2+信号传递反应。在谷氨酸存在的情况下进行机械刺激，会通过因谷氨酸而经历异步Ca2+振荡的细胞诱导典型的Ca2+信号传递波。Ca2+信号传递波与异步Ca2+振荡的共存表明细胞内和细胞间Ca2+信号传导存在不同机制。这种细胞间信号传导可能协调神经胶质细胞的协同功能。
