Matsukura T, Sugase M
Department of Virology II, National Institute of Health, Tokyo, Japan.
Int J Cancer. 1995 Mar 29;61(1):13-22. doi: 10.1002/ijc.2910610104.
We have established a critical identification method for the full spectrum of genital human papillomaviruses (HPVs) in clinical specimens. It was based on the recognition of PstI, BanI and MspI cleavage patterns of HPV DNA detected by blot hybridization with HPV 58 DNA probe at Tm -40 degrees C. By this method, we identified 24 different types of genital HPV including 5 novel types (HPV 59, 61, 62, 64 and 67) in the specimens collected at one hospital and found almost all the HPVs with the authentic cleavage patterns of their respective prototypes. In 235 cervical biopsy specimens, HPV 6 or 11 was found in exophytic condyloma acuminatum (15/15) but not in any cervical intraepithelial neoplasia (CIN) specimens. In contrast, HPV 18, 30, 43, 54, 56, 59, 62, 66 and 67 were identified in CIN I (28/71) or II (4/56) but not in CIN III, while HPV 16, 31, 33, 35, 39, 51, 52 and 58 were identified in CIN III (83/93) as well as in CIN I (34/71) and II (47/56). The result indicates that heterogeneous genital HPVs prevail all over the world. In addition, HPV 6 and 11 are etiologic agents only of exophytic condyloma, whereas the other HPVs are etiologic agents of CIN with the segregation of specific HPVs in CIN III. We propose a new clinicopathologic grouping of genital HPVs founded on nucleotide homology of the HPV genome.
我们已经建立了一种针对临床标本中全谱系生殖道人类乳头瘤病毒（HPV）的关键鉴定方法。该方法基于在Tm -40℃下，通过与HPV 58 DNA探针进行印迹杂交来识别HPV DNA的PstI、BanI和MspI切割模式。通过这种方法，我们在一家医院收集的标本中鉴定出24种不同类型的生殖道HPV，包括5种新型（HPV 59、61、62、64和67），并且发现几乎所有HPV都具有其各自原型的真实切割模式。在235份宫颈活检标本中，在外生性尖锐湿疣（15/15）中发现了HPV 6或11，但在任何宫颈上皮内瘤变（CIN）标本中均未发现。相反，在CIN I（28/71）或II（4/56）中鉴定出了HPV 18、30、43、54、56、59、62、66和67，但在CIN III中未发现，而在CIN III（83/93）以及CIN I（34/71）和II（47/56）中鉴定出了HPV 16、31、33、35、39、51、52和58。结果表明，世界各地普遍存在异质性生殖道HPV。此外，HPV 6和11仅是外生性尖锐湿疣的病原体，而其他HPV是CIN的病原体，且特定HPV在CIN III中有分离现象。我们基于HPV基因组的核苷酸同源性提出了一种新的生殖道HPV临床病理分组方法。
