Penault-Llorca F, Bertucci F, Adélaïde J, Parc P, Coulier F, Jacquemier J, Birnbaum D, deLapeyrière O
Laboratoire de Biologie des Tumeurs, Institut Paoli-Calmettes, Marseille, France.
Int J Cancer. 1995 Apr 10;61(2):170-6. doi: 10.1002/ijc.2910610205.
The family of FGF growth factors is involved in several biological processes and might play an important role in tumorigenesis. We have studied the respective expression of 8 of the 9 characterized FGF genes, and of the 4 known FGF receptor genes, in a panel of 10 tumor-cell lines and 103 breast-tumor samples, using RT-PCR and Northern-blot analyses. FGF1 and FGF2 were expressed in almost all samples, while expression of FGF5, FGF6, FGF7, and FGF9 was more restricted. FGFR1, FGFR2 and FGFR4 were expressed at high levels in respectively 22%, 4% and 32% of tumors. FGFR3 expression was not detected. The transcript encoding an FGFR1 isoform with 2 immunoglobulin-like domains was the most prevalent.
FGF生长因子家族参与多种生物学过程，可能在肿瘤发生中发挥重要作用。我们使用逆转录聚合酶链反应（RT-PCR）和Northern印迹分析，研究了9个已鉴定的FGF基因中的8个以及4个已知FGF受体基因在一组10个肿瘤细胞系和103个乳腺肿瘤样本中的各自表达情况。FGF1和FGF2在几乎所有样本中均有表达，而FGF5、FGF6、FGF7和FGF9的表达则更为局限。FGFR1、FGFR2和FGFR4分别在22%、4%和32%的肿瘤中高水平表达。未检测到FGFR3的表达。编码具有2个免疫球蛋白样结构域的FGFR1异构体的转录本最为普遍。
