Cenci G, Bonaccorsi S, Pisano C, Verni F, Gatti M
Istituto Pasteur, Fondazione Cenci Bolognetti, Roma, Italy.
J Cell Sci. 1994 Dec;107 ( Pt 12):3521-34. doi: 10.1242/jcs.107.12.3521.
Larval and pupal testes of Drosophila melanogaster were fixed with a methanol/acetone fixation procedure that results in good preservation of cell morphology; fixed cells viewed by phase-contrast optics exhibit most of the structural details that can be seen in live material. Fixed testis preparations were treated with anti-tubulin antibodies and Hoechst 33258 to selectively stain microtubules and DNA. The combined analysis of cell morphology, chromatin and microtubule organization allowed a fine cytological dissection of gonial cell multiplication, spermatocyte development, meiosis and the early stages of spermatid differentiation. We placed special emphasis on the spermatocyte growth phase and the meiotic divisions, providing a description of these processes that is much more detailed than those previously reported. In addition, by means of bromo-deoxyuridine incorporation experiments, we were able to demonstrate that premeiotic DNA synthesis occurs very early during spermatocyte growth.
黑腹果蝇的幼虫和蛹期睾丸用甲醇/丙酮固定法进行固定，该方法能很好地保存细胞形态；用相差光学显微镜观察固定后的细胞，能呈现出在活材料中可见的大部分结构细节。将固定好的睾丸制剂用抗微管蛋白抗体和Hoechst 33258处理，以选择性地对微管和DNA进行染色。通过对细胞形态、染色质和微管组织的综合分析，能够对生殖细胞增殖、精母细胞发育、减数分裂以及精子细胞分化的早期阶段进行精细的细胞学剖析。我们特别关注精母细胞生长阶段和减数分裂，对这些过程的描述比之前报道的更为详细。此外，通过溴脱氧尿苷掺入实验，我们能够证明减数分裂前的DNA合成在精母细胞生长过程中很早就发生了。
