Cassone A, De Bernardis F, Pontieri E, Carruba G, Girmenia C, Martino P, Fernández-Rodríguez M, Quindós G, Pontón J
Department of Bacteriology and Medical Mycology, Istituto Superiore di Sanità, Rome, Italy.
J Infect Dis. 1995 Apr;171(4):967-75. doi: 10.1093/infdis/171.4.967.
Environmental, vaginal, and blood isolates of Candida parapsilosis were biotyped by karyotype analysis in pulsed-field gel electrophoresis. Morphotype and resistotype were also determined as was aspartyl proteinase secretion and pathogenicity in a systemic mouse infection model. Overall, the karyotype patterns consisted of 6-9 chromosome bands (> 3.0-0.6 Mb) with limited clustering, since most isolates had unique chromosome profiles. Major clusters C. parapsilosis, differing by source of isolation and in experimental pathogenicity, could be discriminated by morphoresistotyping. The morphotypes of isolates from subjects with candidemia ranged from one that caused elevated mortality in the normal mouse to those that were totally avirulent in the neutropenic animal. Among clinical isolates, secretion of aspartyl proteinase was higher in vaginitis than in candidemia isolates and did not correlate with the experimental pathogenicity. These results emphasize the biotype diversity of C. parapsilosis and have potentially important epidemiologic and pathologic implications.
通过脉冲场凝胶电泳中的核型分析对近平滑念珠菌的环境、阴道和血液分离株进行生物分型。还确定了形态型和耐药型，以及天冬氨酸蛋白酶分泌情况和在系统性小鼠感染模型中的致病性。总体而言，核型模式由6 - 9条染色体带（> 3.0 - 0.6 Mb）组成，聚类有限，因为大多数分离株具有独特的染色体图谱。通过形态耐药分型可以区分主要的近平滑念珠菌聚类，它们因分离来源和实验致病性不同而有所差异。念珠菌血症患者分离株的形态型范围从在正常小鼠中导致死亡率升高的菌株到在中性粒细胞减少动物中完全无致病性的菌株。在临床分离株中，阴道炎分离株中天冬氨酸蛋白酶的分泌高于念珠菌血症分离株，且与实验致病性无关。这些结果强调了近平滑念珠菌的生物型多样性，并具有潜在重要的流行病学和病理学意义。
