Moreno-Martínez Ana Esther, Gómez-Molero Emilia, Sánchez-Virosta Pablo, Dekker Henk L, de Boer Albert, Eraso Elena, Bader Oliver, de Groot Piet W J
Albacete Regional Center for Biomedical Research, Castilla-La Mancha Science & Technology Park, University of Castilla-La Mancha, 02008 Albacete, Spain.
Institute for Medical Microbiology, University Medical Center Göttingen, Kreuzbergring 57, 37075 Göttingen, Germany.
Pathogens. 2021 Apr 19;10(4):493. doi: 10.3390/pathogens10040493.
is among the most frequent causes of candidiasis. Clinical isolates of this species show large variations in colony morphotype, ranging from round and smooth to a variety of non-smooth irregular colony shapes. A non-smooth appearance is related to increased formation of pseudohyphae, higher capacity to form biofilms on abiotic surfaces, and invading agar. Here, we present a comprehensive study of the cell wall proteome of reference strain CDC317 and seven clinical isolates under planktonic and sessile conditions. This analysis resulted in the identification of 40 wall proteins, most of them homologs of known cell wall proteins, such as Gas, Crh, Bgl2, Cht2, Ecm33, Sap, Sod, Plb, Pir, Pga30, Pga59, and adhesin family members. Comparative analysis of exponentially growing and stationary phase planktonic cultures of CDC317 at 30 °C and 37 °C revealed only minor variations. However, comparison of smooth isolates to non-smooth isolates with high biofilm formation capacity showed an increase in abundance and diversity of putative wall adhesins from Als, Iff/Hyr, and Hwp families in the latter. This difference depended more strongly on strain phenotype than on the growth conditions, as it was observed in planktonic as well as biofilm cells. Thus, in the set of isolates analyzed, the high biofilm formation capacity of non-smooth isolates with elongated cellular phenotypes correlates with the increased surface expression of putative wall adhesins in accordance with their proposed cellular function.
是念珠菌病最常见的病因之一。该菌种的临床分离株在菌落形态型上表现出很大差异，从圆形光滑到各种非光滑不规则菌落形状都有。非光滑外观与假菌丝形成增加、在非生物表面形成生物膜的能力增强以及侵入琼脂有关。在这里，我们对参考菌株CDC317和七株临床分离株在浮游和固着条件下的细胞壁蛋白质组进行了全面研究。该分析鉴定出40种壁蛋白，其中大多数是已知细胞壁蛋白的同源物，如Gas、Crh、Bgl2、Cht2、Ecm33、Sap、Sod、Plb、Pir、Pga30、Pga59和粘附素家族成员。对CDC317在30℃和37℃下指数生长和稳定期浮游培养物的比较分析仅显示出微小差异。然而，将光滑分离株与具有高生物膜形成能力的非光滑分离株进行比较，发现后者中来自Als、Iff/Hyr和Hwp家族的假定壁粘附素的丰度和多样性增加。这种差异更多地取决于菌株表型而非生长条件，因为在浮游细胞和生物膜细胞中均观察到。因此，在所分析的分离株组中，具有细长细胞表型的非光滑分离株的高生物膜形成能力与其假定壁粘附素的表面表达增加相关，这与其提出的细胞功能一致。
