Goda F, Liu K J, Walczak T, O'Hara J A, Jiang J, Swartz H M
First Department of Surgery, Kagawa Medical School, Japan.
Magn Reson Med. 1995 Feb;33(2):237-45. doi: 10.1002/mrm.1910330214.
Recent advances in electron paramagnetic resonance (EPR) oximetry have established the use of the particulate materials fusinite and lithium phthalocyanine (LiPc) as probes for measurement of oxygen tensions in tissues in vivo with a sensitivity and accuracy that is relevant for studying pathophysiological processes. India ink, another potentially very useful new probe for EPR oximetry, shares the critical properties of fusinite and LiPc and has the additional advantage of already having been widely used clinically with no apparent toxicity. The line width is particularly sensitive to changes in oxygen tension of less than 30 mmHg; in this range the line broadening/unit of pO2 is much greater than that of fusinite and LiPc. Over the range of biological conditions that can occur in vivo, the response of the EPR spectrum of India ink to pO2 is independent of pH, oxidants, reductants, and the nature of the medium. In this paper we describe the relevant properties of India ink and its use to measure pO2 in vivo in experimental animals and a human subject.
电子顺磁共振（EPR）血氧测定法的最新进展已确立了使用微粒材料丝炭和锂酞菁（LiPc）作为探针来测量体内组织中的氧张力，其灵敏度和准确性与研究病理生理过程相关。印度墨水是EPR血氧测定法另一种潜在的非常有用的新型探针，它具有丝炭和LiPc的关键特性，并且还有一个额外的优点，即已在临床上广泛使用且无明显毒性。线宽对低于30 mmHg的氧张力变化特别敏感；在此范围内，每单位pO2的线宽展宽比丝炭和LiPc大得多。在体内可能出现的生物条件范围内，印度墨水的EPR光谱对pO2的响应与pH、氧化剂、还原剂和介质性质无关。在本文中，我们描述了印度墨水的相关特性及其在实验动物和人类受试者体内测量pO2的用途。
