EPR Center for the Study of Viable Systems, The Geisel School of Medicine at Dartmouth, Lebanon, NH, 03766, USA.
EPR Center for the Study of Viable Systems, Department of Radiology, Norris Cotton Cancer Center, Geisel School of Medicine, Dartmouth College, 48 Lafayette Street, HB 7785, Lebanon, NH, 03766, USA.
Adv Exp Med Biol. 2014;812:73-79. doi: 10.1007/978-1-4939-0620-8_10.
EPR oximetry, which enables reliable, accurate, and repeated measurements of the partial pressure of oxygen in tissues, provides a unique opportunity to investigate the role of oxygen in the pathogenesis and treatment of several diseases including cancer, stroke, and heart failure. Building on significant advances in the in vivo application of EPR oximetry for small animal models of disease, we are developing suitable probes and instrumentation required for use in human subjects. Our laboratory has established the feasibility of clinical EPR oximetry in cancer patients using India ink, the only material presently approved for clinical use. We now are developing the next generation of probes, which are both superior in terms of oxygen sensitivity and biocompatibility including an excellent safety profile for use in humans. Further advances include the development of implantable oxygen sensors linked to an external coupling loop for measurements of deep-tissue oxygenations at any depth, overcoming the current limitation of 10 mm. This paper presents an overview of recent developments in our ability to make meaningful measurements of oxygen partial pressures in human subjects under clinical settings.
EPR 血氧计可可靠、准确、重复地测量组织中的氧分压，为研究氧气在癌症、中风和心力衰竭等多种疾病的发病机制和治疗中的作用提供了独特的机会。基于 EPR 血氧计在疾病小动物模型中的体内应用的重大进展，我们正在开发适用于人体的合适探头和仪器。我们的实验室已经使用目前唯一批准临床使用的印度墨水在癌症患者中证明了临床 EPR 血氧计的可行性。我们现在正在开发下一代探头，在氧灵敏度和生物相容性方面都更优越，包括在人体中使用的出色安全性。进一步的进展包括开发与外部耦合环相连的可植入氧传感器，以测量任何深度的深部组织氧合，克服目前 10 毫米的限制。本文介绍了我们在临床环境下对人体氧分压进行有意义测量的能力的最新进展概述。
