Van Buiten Charlene B, Seitz Valerie A, Metcalf Jessica L, Raskin Ilya
Department of Food Science and Human Nutrition, College of Health and Human Sciences, Colorado State University, Fort Collins, CO 80525, USA.
Department of Animal Sciences, College of Agricultural Sciences, Colorado State University, Fort Collins, CO 80525, USA.
Antioxidants (Basel). 2024 Feb 29;13(3):304. doi: 10.3390/antiox13030304.
Obesity and metabolic dysfunction have been shown to be associated with overproduction of reactive oxygen species (ROS) in the gastrointestinal (GI) tract, which contributes to dysbiosis or imbalances in the gut microbiota. Recently, the reversal of dysbiosis has been observed as a result of dietary supplementation with antioxidative compounds including polyphenols. Likewise, dietary polyphenols have been associated with scavenging of GI ROS, leading to the hypothesis that radical scavenging in the GI tract is a potential mechanism for the reversal of dysbiosis. The objective of this study was to investigate the relationship between GI ROS, dietary antioxidants and beneficial gut bacterium . The results of this study demonstrated to be a discriminant microorganism between lean ( = 7) and obese ( = 7) mice. The relative abundance of was also found to have a significant negative correlation with extracellular ROS in the GI tract as measured using fluorescent probe hydroindocyanine green. The ability of the dietary antioxidants ascorbic acid, β-carotene and grape polyphenols to scavenge GI ROS was evaluated in tandem with their ability to support bloom in lean mice ( = 20). While the relationship between GI ROS and relative abundance of was conserved in lean mice, only grape polyphenols stimulated the bloom of . Analysis of fecal antioxidant capacity and differences in the bioavailability of the antioxidants of interest suggested that the poor bioavailability of grape polyphenols contributes to their superior radical scavenging activity and support of in comparison to the other compounds tested. These findings demonstrate the utility of the GI redox environment as a modifiable therapeutic target in the treatment of chronic inflammatory diseases like metabolic syndrome.
肥胖和代谢功能障碍已被证明与胃肠道（GI）中活性氧（ROS）的过量产生有关，这会导致肠道微生物群失调或失衡。最近，人们观察到，通过补充包括多酚在内的抗氧化化合物进行饮食干预，可使失调的微生物群恢复正常。同样，膳食多酚与清除胃肠道ROS有关，这导致了一种假说，即胃肠道中的自由基清除是微生物群失调恢复的潜在机制。本研究的目的是调查胃肠道ROS、膳食抗氧化剂与有益肠道细菌之间的关系。本研究结果表明，[具体细菌名称]是瘦小鼠（n = 7）和肥胖小鼠（n = 7）之间的一种判别微生物。还发现，使用荧光探针氢吲哚菁绿测量时，[具体细菌名称]的相对丰度与胃肠道中的细胞外ROS呈显著负相关。同时评估了膳食抗氧化剂抗坏血酸、β-胡萝卜素和葡萄多酚清除胃肠道ROS的能力，以及它们在瘦小鼠（n = 20）中支持[具体细菌名称]繁殖的能力。虽然在瘦小鼠中，胃肠道ROS与[具体细菌名称]相对丰度之间的关系保持不变，但只有葡萄多酚能刺激[具体细菌名称]的繁殖。对粪便抗氧化能力以及相关抗氧化剂生物利用度差异的分析表明，与其他测试化合物相比，葡萄多酚较差的生物利用度有助于其卓越的自由基清除活性以及对[具体细菌名称]的支持作用。这些发现证明了胃肠道氧化还原环境作为治疗慢性炎症性疾病（如代谢综合征）的可调节治疗靶点的效用。
