Melcer T, Gonzalez D, Riley E P
Department of Psychology, San Diego State University, CA 92120.
Neurotoxicol Teratol. 1995 Jan-Feb;17(1):41-8. doi: 10.1016/0892-0362(94)00051-e.
Recently, we reported that the alcohol preferring (P) and nonpreferring (NP) rats, bred for differences in alcohol preference, showed different behavioral effects of neonatal alcohol exposure when tested as juveniles. Following neonatal alcohol exposure, the P line showed a greater increase in activity than the NP line relative to their respective controls. In the present study, P and NP rat pups were separated from their mothers and artificially reared from postnatal day (PND) 4 until PND 12. Pups were implanted with intragastric cannulas on PND 4 and fed a stock milk solution every 2 h via an indwelling cannula. One group had alcohol added to the milk diet during the 4 daytime feeds at a dose of 6 g/kg/day on PND 4-7 and a dose of 3 g/kg/day on PNDs 8 and 9. One control group was artificially reared and fed an isocaloric milk solution and a second control group was reared normally with a surrogate dam. Rats were tested as adults (80-day-old) for open-field activity and alcohol preference. Neonatal alcohol exposure caused equivalent increases in activity in P and NP rats. In the ethanol preference test, neither the P nor the NP rats showed any effect of neonatal alcohol treatment although there were large line difference in alcohol preference. These data suggest that the increased susceptibility of young P rats to neonatal alcohol exposure, measured by open-field activity, does not extend to adulthood. Furthermore, neonatal alcohol exposure does not appear to alter alcohol preference in either line.
最近，我们报道了因酒精偏好不同而培育的嗜酒（P）大鼠和非嗜酒（NP）大鼠，在幼年期接受测试时，新生期酒精暴露对其行为产生了不同影响。新生期酒精暴露后，与各自的对照组相比，P系大鼠的活动增加幅度大于NP系大鼠。在本研究中，P和NP品系的幼鼠在出生后第4天（PND4）与母亲分离，并从PND4至PND12进行人工饲养。幼鼠在PND4植入胃内插管，并通过留置插管每2小时喂食一次储备乳溶液。一组在白天的4次喂食期间，于PND4 - 7以6 g/kg/天的剂量、PND8和9以3 g/kg/天的剂量在乳类饮食中添加酒精。一个对照组进行人工饲养并喂食等热量的乳溶液，另一个对照组由代孕母鼠正常饲养。将大鼠作为成年鼠（80日龄）进行旷场活动和酒精偏好测试。新生期酒精暴露使P和NP大鼠的活动增加程度相当。在乙醇偏好测试中，尽管P和NP大鼠在酒精偏好上存在较大品系差异，但新生期酒精处理对它们均未产生任何影响。这些数据表明，通过旷场活动测量的幼龄P大鼠对新生期酒精暴露的易感性增加在成年期并未延续。此外，新生期酒精暴露似乎并未改变任一品系的酒精偏好。
