神经节苷脂GD3在中枢神经系统中的发育概况：大鼠的免疫细胞化学研究

Developmental profile of ganglioside GD3 in the central nervous system: an immunocytochemical study in the rat.

作者信息

Kawai K, Kuroda S, Watarai S, Takahashi H

机构信息

Department of Neuropsychiatry, Okayama University Medical School, Japan.

出版信息

Neurosci Res. 1994 Nov;21(1):77-82. doi: 10.1016/0168-0102(94)90070-1.

DOI:10.1016/0168-0102(94)90070-1

PMID:7708295

Abstract

Light and electron microscopy observations were made of developing rat brain at gestation days (E) E13, E16, E19, and postnatal days (P) P1, P3, P5, P7 after immunocytochemical staining for ganglioside GD3 (II3 alpha(NeuAc alpha 2-8NeuAc)-LacCer, GD3) using mouse IgM anti-GD3 monoclonal antibody (DSG-1). Immunoreactivity was observed in neuroblasts (E13, E16) and immature neuronal cells (P1, P3, P5, P7), and also in glioblasts (E19). Electron microscopy revealed that at E13 peroxidase reaction product for GD3 (RP) was present on the plasma membrane and in the cytoplasm of neuroblasts, with accentuation in the former. At E16, RP was observed predominantly in the cytoplasm of neuroblasts. At E19, EP was seen mainly in the cytoplasm of glioblasts. At P1, P3, P5, and P7, immature neuronal cells in the cerebrum were immunoreactive. In the cerebellum, immature Purkinje cells and immature neuronal cells in the external and internal granular layers were also immunoreactive for GD3, the reaction product being located in the cytoplasm. The present findings suggest that changes in the localization of GD3 in neuroblasts were correlated with the alteration in their biological functions.

摘要

使用小鼠IgM抗GD3单克隆抗体（DSG-1）对神经节苷脂GD3（II3α(NeuAcα2-8NeuAc)-LacCer，GD3）进行免疫细胞化学染色后，对妊娠第（E）E13、E16、E19天以及出生后第（P）P1、P3、P5、P7天的发育中大鼠脑进行了光镜和电镜观察。在神经母细胞（E13、E16）和未成熟神经元细胞（P1、P3、P5、P7）中观察到免疫反应性，在成胶质细胞（E19）中也观察到免疫反应性。电镜显示，在E13时，GD3的过氧化物酶反应产物（RP）存在于神经母细胞的质膜和细胞质中，前者更为明显。在E16时，RP主要在神经母细胞的细胞质中观察到。在E19时，EP主要见于成胶质细胞的细胞质中。在P1、P3、P5和P7时，大脑中的未成熟神经元细胞具有免疫反应性。在小脑中，未成熟的浦肯野细胞以及外颗粒层和内颗粒层中的未成熟神经元细胞对GD3也具有免疫反应性，反应产物位于细胞质中。本研究结果表明，神经母细胞中GD3定位的变化与其生物学功能的改变相关。