Matthew E, Andreason P, Pettigrew K, Carson R E, Herscovitch P, Cohen R, King C, Johanson C E, Greenblatt D J, Paul S M
Clinical Neuroscience Branch, National Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892, USA.
Proc Natl Acad Sci U S A. 1995 Mar 28;92(7):2775-9. doi: 10.1073/pnas.92.7.2775.
We studied the effects of a high-affinity gamma-aminobutyric acid (GABA)-benzodiazepine-receptor agonist (lorazepam) and an antagonist (flumazenil) in humans, using H2(15)O positron-emission tomography. Administration of lorazepam to healthy volunteers caused time- and dose-dependent reductions in regional cerebral blood flow and self-reported alterations in behavioral/mood parameters. Flumazenil administration reversed these changes. These observations indicated that benzodiazepine-induced effects on regional cerebral blood flow and mood/behavior are mediated at some level through GABA-benzodiazepine receptors, although the specific mechanism remains unclear. The approach described here provides a method for quantifying GABA-benzodiazepine-receptor-mediated neurotransmission in the living human brain and may be useful for studying the role of these receptors in a variety of neuropsychiatric disorders.
我们使用H2(15)O正电子发射断层扫描技术，研究了高亲和力γ-氨基丁酸（GABA）-苯二氮䓬受体激动剂（劳拉西泮）和拮抗剂（氟马西尼）对人体的影响。给健康志愿者服用劳拉西泮会导致局部脑血流量出现时间和剂量依赖性减少，以及自我报告的行为/情绪参数改变。服用氟马西尼可逆转这些变化。这些观察结果表明，苯二氮䓬对局部脑血流量和情绪/行为的影响在某种程度上是通过GABA-苯二氮䓬受体介导的，尽管具体机制尚不清楚。这里描述的方法提供了一种量化活体人脑中GABA-苯二氮䓬受体介导的神经传递的方法，可能有助于研究这些受体在各种神经精神疾病中的作用。
