Huntley J F, Patterson M, Mackellar A, Jackson F, Stevenson L M, Coop R L
Moredun Research Institute, Edinburgh.
Res Vet Sci. 1995 Jan;58(1):5-10. doi: 10.1016/0034-5288(95)90080-2.
The mucosal mast cell and eosinophil responses of goats and sheep to a mixed gastrointestinal nematode infection were compared. Groups of eight does and nine ewes, previously maintained on pasture and treated with anthelmintic when they were housed and five worm-free lambs were challenged with 10,000 Trichostrongylus vitrinus third stage larvae (L3) and 10,000 Teladorsagia circumcincta L3. Eleven days after challenge, the ewes had significantly (P < 0.001) lower burdens of abomasal and intestinal worms than the does or naive lambs, but significantly higher (P < 0.001) tissue concentrations of mast cell proteinase. Toluidine blue-stained sections indicated a paucity of mast cells in the does compared with the ewes, whereas the immunolocalisation of sheep mast cell proteinase revealed similar numbers of stained cells in the two species. This discrepancy was due to the relatively high proportion of globule leucocytes (77 and 91 per cent in the jejunum and abomasum, respectively) in the does compared with the ewes (7 and 24 per cent in the jejunum and abomasum, respectively). No differences were detected between the numbers of circulating or tissue eosinophils in the ewes and does.
对山羊和绵羊在混合胃肠道线虫感染时的黏膜肥大细胞和嗜酸性粒细胞反应进行了比较。将八只母山羊和九只母绵羊分为一组，它们之前一直在牧场饲养，在圈养时用驱虫药进行过处理，另外五只未感染蠕虫的羔羊用10000条玻璃细颈线虫第三期幼虫（L3）和10000条环形泰勒线虫L3进行攻虫。攻虫11天后，母绵羊皱胃和肠道内蠕虫的负荷量显著低于（P < 0.001）母山羊或未感染的羔羊，但肥大细胞蛋白酶的组织浓度显著高于（P < 0.001）它们。甲苯胺蓝染色切片显示，与母绵羊相比，母山羊体内的肥大细胞较少，而绵羊肥大细胞蛋白酶的免疫定位显示，两个物种中染色细胞的数量相似。这种差异是由于与母绵羊相比，母山羊中球形白细胞的比例相对较高（空肠和皱胃中分别为77%和91%），而母绵羊空肠和皱胃中的球形白细胞比例分别为7%和24%。在母绵羊和母山羊中，循环或组织嗜酸性粒细胞的数量未检测到差异。
