Stevenson L M, Huntley J F, Smith W D, Jones D G
Moredun Research Institute, Edinburgh, UK.
FEMS Immunol Med Microbiol. 1994 Feb;8(2):167-73. doi: 10.1111/j.1574-695X.1994.tb00439.x.
Eosinophil numbers in peripheral blood and eosinophil potentiating activity (EPA) and sheep mast cell protease (SMCP) in efferent gastric lymph were monitored in lambs during infections with Ostertagia circumcincta. Worm burdens, eosinophil numbers in bone marrow, abomasal mucosa and gastric lymph node, as well as mast cell numbers and SMCP concentrations in mucosa and mucus, were determined in post mortem samples. In naive lambs, high and relatively uniform worm burdens were present 10 days after primary infection and these were associated with only mild blood and tissue eosinophilia. By day 21 worm burdens were markedly lower and more variable. There was more evidence of eosinophil and mast cell accumulation in mucosa, and numbers in bone marrow were also higher than on day 10. However, neither EPA nor SMCP were detectable in lymph. By contrast, EPA and SMCP were present in substantial amounts in draining lymph within 48 h of challenge (secondary) infection of previously exposed lambs. EPA was inversely related to worm burdens recovered on day 10, as were abomasal mucosal and mucus SMCP concentrations. Elevated eosinophil numbers were also consistently detected in blood, bone marrow, mucosa and gastric lymph node. The results suggest that host immune defence against secondary, but not primary, exposure to O. circumcincta involves a rapidly mobilised local inflammatory component.
在感染环纹奥斯特线虫的羔羊中，监测外周血中的嗜酸性粒细胞数量以及胃传出淋巴中的嗜酸性粒细胞增强活性（EPA）和绵羊肥大细胞蛋白酶（SMCP）。在死后样本中测定虫负荷、骨髓、皱胃黏膜和胃淋巴结中的嗜酸性粒细胞数量，以及黏膜和黏液中的肥大细胞数量和SMCP浓度。在未感染的羔羊中，初次感染10天后出现高且相对均匀的虫负荷，这些仅与轻度的血液和组织嗜酸性粒细胞增多有关。到第21天，虫负荷明显降低且更具变异性。有更多证据表明黏膜中有嗜酸性粒细胞和肥大细胞积聚，骨髓中的数量也高于第10天。然而，在淋巴中未检测到EPA和SMCP。相比之下，在先前暴露的羔羊受到攻击（二次）感染后48小时内，引流淋巴中大量存在EPA和SMCP。EPA与第10天回收的虫负荷呈负相关，皱胃黏膜和黏液中的SMCP浓度也是如此。在血液、骨髓、黏膜和胃淋巴结中也持续检测到嗜酸性粒细胞数量升高。结果表明，宿主针对二次而非初次接触环纹奥斯特线虫的免疫防御涉及快速动员的局部炎症成分。
