González R D, Oberti E R
Laboratorio de Microbiología, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Argentina.
Rev Latinoam Microbiol. 1994 Jul-Sep;36(3):177-81.
A Moraxella bovis strain was isolated from a kerato-conjunctivities lesion of a calf in Villa Valeria (Córdoba); it was used to establish improved cultural conditions, such as nature and concentration of carbon and nitrogen sources, and pH control in shaken flasks. The selected conditions were assayed for biomass production in a bench-top fermentor. The strain is used by the pharmaceutical industry to produce vaccines and adjuvants. In the initial condition (48 h culture on blood agar) 0.019 g biomass/l.h-1 was obtained. With the use of liquid defined medium with pH control, productivity was increased to 0.153g/l.h-1, with optimum harvest time of 32 h.
从科尔多瓦省巴列里亚镇一头小牛的角膜结膜炎损伤处分离出一株牛莫拉菌；用它来确定改良的培养条件，如碳源和氮源的性质及浓度，以及摇瓶中的pH控制。对选定的条件在台式发酵罐中进行生物量生产测定。该菌株被制药行业用于生产疫苗和佐剂。在初始条件下（在血琼脂上培养48小时），生物量产量为0.019克/升·小时-1。使用带pH控制的液体限定培养基后，生产率提高到0.153克/升·小时-1，最佳收获时间为32小时。
