Boyer M I, Bowen C V, Danska J S
Division of Surgical Research, Hospital for Sick Children Research Institute, Toronto, Canada.
Transplantation. 1995 Apr 15;59(7):1043-6. doi: 10.1097/00007890-199504150-00022.
As a model system, rabbits are particularly useful in transplantation studies because of their size and accessibility. However, inbred rabbits of known MHC haplotype are not commercially available, and there are few monoclonal antibody reagents available to permit serological typing of experimental animals prior to transplant. Here we present a rapid and reliable method to distinguish rabbits that differ at their MHC class I and class II loci, and present 34 class I and 16 class II haplotypes determined by restriction fragment-length polymorphism analysis of outbred heterozygous rabbits. The applicability of this molecular typing system to transplantation experiments in the New Zealand White rabbit is discussed.
作为一种模型系统，兔子因其体型和易操作性在移植研究中特别有用。然而，具有已知MHC单倍型的近交系兔子没有商业供应，并且在移植前用于对实验动物进行血清学分型的单克隆抗体试剂很少。在这里，我们提出了一种快速可靠的方法来区分在MHC I类和II类基因座上存在差异的兔子，并展示了通过对远交杂合兔子进行限制性片段长度多态性分析确定的34种I类和16种II类单倍型。讨论了这种分子分型系统在新西兰白兔移植实验中的适用性。
