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鸡B-F和B-L基因的限制性片段长度多态性分析及其与血清学定义的B单倍型的关联。

Restriction fragment length polymorphism analysis of the chicken B-F and B-L genes and their association with serologically defined B haplotypes.

作者信息

Juul-Madsen H R, Hedemand J E, Salomonsen J, Simonsen M

机构信息

Royal Veterinary and Agricultural University, Frederiksberg C., Denmark.

出版信息

Anim Genet. 1993 Aug;24(4):243-7. doi: 10.1111/j.1365-2052.1993.tb00306.x.

DOI:10.1111/j.1365-2052.1993.tb00306.x
PMID:7902038
Abstract

Seven serologically defined chicken haplotypes have been analysed by restriction fragment length polymorphism (RFLP) with chicken cDNA probes specific for MHC class I and II. The results demonstrate an excellent correlation between the observed RFLP banding patterns in the investigated haplotypes and the serological B-typing. In future, RFLP analysis in addition to serological B-typing may sharpen the tools in the search for recombinant chromosomes separating B-F and B-L.

摘要

利用针对MHC I类和II类的鸡cDNA探针,通过限制性片段长度多态性(RFLP)分析了七种血清学定义的鸡单倍型。结果表明,在所研究的单倍型中观察到的RFLP条带模式与血清学B分型之间具有极好的相关性。未来,除血清学B分型外,RFLP分析可能会完善寻找分离B-F和B-L的重组染色体的工具。

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