用茚三酮-铁试剂测定赖氨酸。
Determination of lysine with ninhydrin-ferric reagent.
作者信息
Hsieh C L, Hsiung K P, Su J C
机构信息
Union Chemical Laboratories, Industrial Technology Research Institute, Hsinchu, Taiwan.
出版信息
Anal Biochem. 1995 Jan 1;224(1):187-9. doi: 10.1006/abio.1995.1027.
PMID:7710069
Abstract
Ninhydrin-ferric reagent reacts highly and specifically with lysine at a pH value of 1.0. Ferric ion inhibits the reaction of ninhydrin with proline, ornithine, glycine, arginine, and histidine. This method can be used to determine a lysine sample of high concentration without dilution. It has a linear response range from 0.0625 to 0.5 mg lysine.HCl.
摘要
茚三酮 - 铁试剂在pH值为1.0时与赖氨酸发生高度特异性反应。铁离子会抑制茚三酮与脯氨酸、鸟氨酸、甘氨酸、精氨酸和组氨酸的反应。该方法可用于直接测定高浓度赖氨酸样品，无需稀释。其线性响应范围为0.0625至0.5mg赖氨酸·盐酸盐。
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