GCR1是酿酒酵母糖酵解基因的转录激活因子，其突变可作为gcr2突变的抑制因子发挥作用。
Mutations in GCR1, a transcriptional activator of Saccharomyces cerevisiae glycolytic genes, function as suppressors of gcr2 mutations.
作者信息
Uemura H, Jigami Y
机构信息
Department of Molecular Biology, Tsukuba Research Center (MITI), Ibaraki, Japan.
出版信息
Genetics. 1995 Feb;139(2):511-21. doi: 10.1093/genetics/139.2.511.
Abstract
The Saccharomyces cerevisiae GCR1 and GCR2 genes affect expression of most of the glycolytic genes. Evidence for Gcr1p/Gcr2p interaction has been presented earlier and is now supported by the isolation of mutations in Gcr1p suppressing gcr2, as assessed by growth and enzyme assay. Four specific mutation sites were identified. Together with use of the two-hybrid system of Fields and Song, they show that Gcr1p in its N-terminal half has a potential transcriptional activating function as well as elements for interaction with Gcr2p, which perhaps acts normally to expose an otherwise cryptic activation domain on Gcr1p. Complementation of various gcr1 mutant alleles and results with the two-hybrid system also indicate that Gcr1p itself normally functions as an oligomer.
摘要
酿酒酵母的GCR1和GCR2基因影响大多数糖酵解基因的表达。先前已有关于Gcr1p/Gcr2p相互作用的证据，现在通过生长和酶活性测定评估，发现Gcr1p中抑制gcr2的突变体的分离也支持了这一点。确定了四个特定的突变位点。结合使用Fields和Song的双杂交系统，结果表明Gcr1p的N端一半具有潜在的转录激活功能以及与Gcr2p相互作用的元件，Gcr2p可能正常发挥作用以暴露Gcr1p上原本隐藏的激活结构域。各种gcr1突变等位基因的互补以及双杂交系统的结果还表明，Gcr1p本身通常作为寡聚体发挥作用。
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