在pH 6条件下感染产生的小鼠冠状病毒刺突糖蛋白的病毒滴度增加，且抗体反应性增强。
Increased viral titers and enhanced reactivity of antibodies to the spike glycoprotein of murine coronavirus produced by infection at pH 6.
作者信息
Daniel C, Lamarre A, Talbot P J
机构信息
Virology Research Center, Institut Armand-Frappier, Université du Québec, Laval, Canada.
出版信息
J Virol Methods. 1994 Dec;50(1-3):237-44. doi: 10.1016/0166-0934(94)90180-5.
Abstract
Infection of cell monolayers by murine coronavirus A59 at pH 6 rather than 7 yielded a ten-fold increase in the infectious titer and a remarkable enhancement of the reactivities of monoclonal and polyclonal antibodies against the spike glycoprotein in immunoblotting, immunoprecipitation and enzyme-linked immunosorbent assays. These observations are very useful for detecting antibodies against the S glycoprotein of coronaviruses and enhancing infectious titers.
摘要
鼠冠状病毒A59在pH 6而非pH 7条件下感染细胞单层，可使感染滴度提高10倍，并在免疫印迹、免疫沉淀和酶联免疫吸附测定中显著增强针对刺突糖蛋白的单克隆和多克隆抗体的反应性。这些观察结果对于检测针对冠状病毒S糖蛋白的抗体以及提高感染滴度非常有用。
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