Sakuma T, Nishimura T, Usuda K, Handa M, Okaniwa G, Nakada T, Tabata T, Hoshikawa Y, Fujimura S
Department of Surgery, Sendai Kosei Hospital, Japan.
Nihon Kyobu Geka Gakkai Zasshi. 1995 Feb;43(2):153-8.
We determined whether activity of neutrophil elastase increases in pleural effusion after lobectomy for the neoplasm in the lung. Samples of pleural effusion were obtained 3 and 24 h postoperatively and samples of the peripheral blood were obtained preoperatively, 3 h, 24 h, and 1 w postoperatively, then the neutrophil counts, the levels of neutrophil elastase and alpha 1-antitrypsin by enzyme-linked immunosorbent assay, the activity of neutrophil elastase using succinyl-(L-alanine) 3-p-nitroanilide were measured. We found that the levels of neutrophil elastase increased 170 times greater in pleural effusion than in peripheral blood 3 h after lobectomy. However, the levels of alpha 1-antitrypsin did not increase in pleural effusion. The activity of neutrophil elastase increased to 0.54 +/- 0.1 mumol/min/l in pleural effusion 3 h after lobectomy, however the activity of neutrophil elastase was under the sensitivity level of detection in the peripheral blood after lobectomy. In conclusion, there is an imbalance between the levels of neutrophil elastase and alpha 1-antitrypsin in pleural effusion after lobectomy, the imbalance resulting in the increased activity of neutrophil elastase which probably is relating to tissue injury in the pleural spaces.
我们确定了肺肿瘤肺叶切除术后胸腔积液中中性粒细胞弹性蛋白酶的活性是否增加。术后3小时和24小时获取胸腔积液样本，术前、术后3小时、24小时和1周获取外周血样本，然后通过酶联免疫吸附测定法测量中性粒细胞计数、中性粒细胞弹性蛋白酶和α1 -抗胰蛋白酶水平，使用琥珀酰 -（L -丙氨酸）3 -对硝基苯胺测量中性粒细胞弹性蛋白酶的活性。我们发现肺叶切除术后3小时胸腔积液中中性粒细胞弹性蛋白酶水平比外周血中高170倍。然而，胸腔积液中α1 -抗胰蛋白酶水平并未升高。肺叶切除术后3小时胸腔积液中中性粒细胞弹性蛋白酶活性增至0.54±0.1μmol/分钟/升，然而肺叶切除术后外周血中中性粒细胞弹性蛋白酶活性低于检测灵敏度水平。总之，肺叶切除术后胸腔积液中中性粒细胞弹性蛋白酶和α1 -抗胰蛋白酶水平失衡，这种失衡导致中性粒细胞弹性蛋白酶活性增加，这可能与胸腔内组织损伤有关。
