DNA-赖氨酸光共轭物中胸腺嘧啶-赖氨酸和胞嘧啶-赖氨酸加合物的形成。

Formation of thymine-lysine and cytosine-lysine adducts in DNA-lysine photoconjugate.

作者信息

Islam N, Ali R

机构信息

Department of Biochemistry, Faculty of Medicine, A.M.U., Aligarh, India.

出版信息

J Photochem Photobiol B. 1995 Feb;27(2):109-15. doi: 10.1016/1011-1344(94)07062-s.

DOI:10.1016/1011-1344(94)07062-s

PMID:7714671

Abstract

Lysine was covalently conjugated to calf thymus DNA by irradiation with UV light (wavelength, 253.7 nm). The results showed monofunctional covalent photobinding of lysine molecules with bases in DNA. Only the epsilon-amino group of lysine participated in the photoconjugation reaction. Thymine and cytosine were modified by 60% and 25% respectively. The kinetics of the DNA-lysine photoreaction showed that one lysine molecule was in the photobound state per 10, 6, 5 and 4 nucleotide base pairs of DNA on irradiation for 20, 30 40 and 60 min respectively.

摘要

通过紫外线（波长253.7纳米）照射，使赖氨酸与小牛胸腺DNA共价结合。结果表明，赖氨酸分子与DNA中的碱基发生了单功能共价光结合。只有赖氨酸的ε-氨基参与了光共轭反应。胸腺嘧啶和胞嘧啶分别有60%和25%被修饰。DNA-赖氨酸光反应的动力学表明，在分别照射20、30、40和60分钟时，每10、6、5和4个核苷酸碱基对的DNA中有一个赖氨酸分子处于光结合状态。