Grover P K, Resnick M I
Department of Urology, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA.
J Urol. 1995 May;153(5):1716-21.
Two-thirds of matrix of all urinary stones consists of proteins. Despite intense research, their relationship to calculogenesis remains controversial. In an attempt to study excretion of proteins in stone formers, their urinary profiles were analyzed and compared with those of healthy subjects. Two-dimensional gel electrophoresis was employed to obtain high resolution separation of proteins. The urine of patients with histories of idiopathic calcium oxalate (CaOx) calculi contained 7 unique proteins, and 2 others that appeared to be overexpressed. Except for alpha 1-acid glycoprotein, the remaining 8 proteins are previously unknown constituents of urine with molecular weights of approximately 43, 39.5, 29, 26, 25.5, 26.5, 27 and 18.5 kD. Their isoelectric points range from 5.5 to 8.0. Coelectrophoresis of pooled urinary proteins of male and female stone formers disclosed that all 9 proteins had identical charges and molecular weights, regardless of the donors' sex. Analyses of urines of idiopathic recurrent CaOx stone formers who had no radiologically detectable calculi also revealed the presence of these proteins. This excludes the possibility that the proteins might be a consequence of abrasion of urothelial lining by the developing stone(s). Recently defective Tamm-Horsfall mucoprotein (THM) has been implicated in urinary stone formation. Coelectrophoresis of pooled urinary proteins of healthy subjects and stone formers denoted that it had an identical charge and molecular weight in both groups. This suggested that stone formation could not be ascribed to a difference in composition of THM. Whether this is attributable to a dissimilar amino acid sequence of this mucoprotein, remains to be probed.
所有尿路结石基质的三分之二由蛋白质组成。尽管进行了深入研究，它们与结石形成的关系仍存在争议。为了研究结石形成者体内蛋白质的排泄情况，分析了他们的尿液图谱并与健康受试者的进行比较。采用二维凝胶电泳来实现蛋白质的高分辨率分离。有特发性草酸钙（CaOx）结石病史患者的尿液中含有7种独特蛋白质，另外还有2种似乎过度表达。除α1-酸性糖蛋白外，其余8种蛋白质是尿液中以前未知的成分，分子量约为43、39.5、29、26、25.5、26.5、27和18.5 kD。它们的等电点范围为5.5至8.0。对男性和女性结石形成者的混合尿液蛋白质进行共电泳显示，所有9种蛋白质的电荷和分子量相同，与供体性别无关。对无放射学可检测结石的特发性复发性CaOx结石形成者的尿液分析也发现了这些蛋白质的存在。这排除了这些蛋白质可能是正在形成的结石对尿路上皮衬里磨损所致的可能性。最近，有缺陷的Tamm-Horsfall粘蛋白（THM）被认为与尿路结石形成有关。健康受试者和结石形成者的混合尿液蛋白质共电泳表明，两组中该蛋白质的电荷和分子量相同。这表明结石形成不能归因于THM组成的差异。这是否归因于这种粘蛋白不同的氨基酸序列，仍有待探究。
