Wisniewski T, Lalowski M, Golabek A, Vogel T, Frangione B
Department of Pathology, New York University Medical Center, New York 10016, USA.
Lancet. 1995 Apr 15;345(8955):956-8. doi: 10.1016/s0140-6736(95)90701-7.
The presence of the apolipoprotein E4 allele has been identified as a major risk factor for late-onset Alzheimer's disease. Apolipoprotein E has also been found immunohistochemically in Alzheimer's disease lesions. We biochemically isolated amyloid beta from senile plaques and found that a carboxyl-terminal fragment (residues 216-299) of apolipoprotein E co-purified. In vitro this fragment from recombinant apolipoprotein E could form amyloid-like fibrils, which were Congo-red positive. Thus senile plaques may contain both amyloid beta and apolipoprotein E amyloid fibrils.
载脂蛋白E4等位基因的存在已被确定为晚发性阿尔茨海默病的主要危险因素。在阿尔茨海默病病变中也通过免疫组织化学方法发现了载脂蛋白E。我们从老年斑中生物化学分离出β淀粉样蛋白，发现载脂蛋白E的一个羧基末端片段（第216 - 299位氨基酸残基）与之共同纯化。在体外，重组载脂蛋白E的这个片段可以形成刚果红阳性的淀粉样样纤维。因此，老年斑可能同时含有β淀粉样蛋白和载脂蛋白E淀粉样纤维。
