Koletzko S, Haisch M, Seeboth I, Braden B, Hengels K, Koletzko B, Hering P
Kinderpoliklinik der Universität München, Germany.
Lancet. 1995 Apr 15;345(8955):961-2. doi: 10.1016/s0140-6736(95)90704-1.
13C-urea breath tests were sampled in 51 subjects with an isotope-selective non-dispersive infrared spectrometer (NDIRS) and compared with the results of conventional isotope-ratio mass-spectrometry (IRMS). Delta-over-baseline values of both methods correlated well (r = 0.976 at 15 min and 0.985 at 30 min, both p < 0.0005). With a cut-off for delta-over-baseline values of 5/1000 (i. e., 5 per thousand), both methods correctly classified all 36 Helicobacter-pylori-negative and all 15 H-pylori-positive subjects. All infected subjects had values over 11/1000 in all samples with both methods. NDIRS is of equal value as IRMS for the diagnosis of H pylori infection. Because NDIRS gives on-line results and is easier and cheaper, we consider NDIRS useful for clinical practice.
使用同位素选择性非分散红外光谱仪（NDIRS）对51名受试者进行了13C-尿素呼气试验，并将结果与传统同位素比值质谱法（IRMS）的结果进行比较。两种方法的增量与基线值相关性良好（15分钟时r = 0.976，30分钟时r = 0.985，p均<0.0005）。以增量与基线值5/1000（即千分之五）为临界值，两种方法对所有36名幽门螺杆菌阴性和所有15名幽门螺杆菌阳性受试者均做出了正确分类。所有感染受试者的所有样本中，两种方法测得的值均超过11/1000。对于幽门螺杆菌感染的诊断，NDIRS与IRMS具有同等价值。由于NDIRS可提供在线结果，且操作更简便、成本更低，我们认为NDIRS对临床实践有用。
