Xu X J, Hao J X, Olsson T, Kristensson K, van der Meide P H, Wiesenfeld-Hallin Z
Department of Laboratory Medical Science and Technology, Karolinska Institute, Huddinge, Sweden.
Neurosci Lett. 1994 Dec 5;182(2):263-6. doi: 10.1016/0304-3940(94)90812-5.
The effect of intrathecal (i.t.) injection of the cytokine interferon-gamma) (IFN-gamma) on the spinal nociceptive flexor reflex was examined in decerebrate, spinalized, unanesthetized rats. IFN-gamma elicited an initial intense, brief facilitation of the flexor reflex followed by a sustained reflex facilitation lasting 40 +/- 5 min (range 20-65 min). The initial and prolonged reflex facilitations by IFN-gamma were partially and totally blocked, respectively, by i.t. pretreatment with nitro-L-arginine-ester, an inhibitor of nitric oxide synthase, at doses which did not influence spinal cord blood flow. Spinal application of IFN-gamma produced powerful and prolonged facilitation of the flexor reflex, possibly reflecting a hyperalgesic action of this cytokine. The facilitatory effect of IFN-gamma was mediated, at least in part, by the activation of the L-arginine-nitric oxide pathway. Thus, IFN-gamma released in the CNS may participate in eliciting pain and hyperalgesia in infectious or neuroinflammatory diseases where there is increased production of this cytokine.
在去大脑、脊髓横断、未麻醉的大鼠中，研究了鞘内注射细胞因子干扰素-γ（IFN-γ）对脊髓伤害性屈肌反射的影响。IFN-γ引起屈肌反射最初的强烈、短暂的易化，随后是持续40±5分钟（范围20 - 65分钟）的反射易化。IFN-γ引起的最初和延长的反射易化分别被鞘内预先用一氧化氮合酶抑制剂硝基-L-精氨酸酯部分和完全阻断，该剂量不影响脊髓血流。脊髓应用IFN-γ产生强大且持久的屈肌反射易化，可能反映了这种细胞因子的痛觉过敏作用。IFN-γ的易化作用至少部分是由L-精氨酸-一氧化氮途径的激活介导的。因此，在中枢神经系统中释放的IFN-γ可能参与在这种细胞因子产生增加的感染性或神经炎症性疾病中引发疼痛和痛觉过敏。
