通过质子核磁共振弛豫揭示小分子渗透到铁蛋白腔内的过程。
Permeation of small molecules into the cavity of ferritin as revealed by proton nuclear magnetic resonance relaxation.
作者信息
Yang D, Nagayama K
机构信息
Nagayama Protein Array Project, ERATO, JRDC, Tsukuba Research Consortium, Japan.
出版信息
Biochem J. 1995 Apr 1;307 ( Pt 1)(Pt 1):253-6. doi: 10.1042/bj3070253.
Abstract
The NMR relaxation technique was used to investigate the permeation of molecules into the cavity of ferritin. Spin-lattice relaxation times in the rotating frame of various probe molecules were measured for solutions of recombinant horse L-apoferritin without iron and horse spleen apoferritin with very small amounts of ferric ions. The results show that molecules larger than the size of the ferritin channels can pass through the channels into the ferritin interior, and that the maximum size of molecules for the permeation is smaller than maltotriose.
摘要
核磁共振弛豫技术被用于研究分子渗透到铁蛋白腔内的情况。对于不含铁的重组马L-脱铁铁蛋白以及含有极少量铁离子的马脾脱铁铁蛋白溶液，测量了各种探针分子在旋转坐标系中的自旋-晶格弛豫时间。结果表明，大于铁蛋白通道尺寸的分子能够通过通道进入铁蛋白内部，并且渗透的分子最大尺寸小于麦芽三糖。
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