Gitter A H, Zenner H P
Department of Neurophysiology, University of Münster, Germany.
Eur Arch Otorhinolaryngol. 1995;252(1):15-9. doi: 10.1007/BF00171434.
Isolated outer hair cells (OHC) of the guinea pig cochlea were exposed to external alternating electric fields parallel to the longitudinal axis of the cells. This resulted in oscillations of the cells' length that were measured photoelectrically using a ratiometric light amplifier. At 5 Hz and elongations up to 300 nm, amplitude of the cell length during oscillation was a linear function of the amplitude of the sinusoidal electric field. When increasing the stimulus frequency up to 32 kHz, OHC length changes followed the stimulus cycle-by-cycle. Oscillations at frequencies above 32 kHz escaped the experimental approach by their small amplitudes and could not be excluded. The frequency dependence of the motile response measured at 5-12,000 Hz had low-pass filter characteristics in cells of the second, third and fourth turns of the cochlea. However, frequency tuning of the motile response was absent in each OHC and systematic differences between different turns were not observed.
将豚鼠耳蜗的孤立外毛细胞（OHC）暴露于与细胞纵轴平行的外部交变电场中。这导致细胞长度发生振荡，使用比率光放大器通过光电方式对其进行测量。在5Hz以及高达300nm的伸长情况下，振荡期间细胞长度的振幅是正弦电场振幅的线性函数。当将刺激频率增加到32kHz时，外毛细胞长度变化逐周期跟随刺激。高于32kHz频率的振荡因其振幅小而超出了实验方法的范围，无法排除。在耳蜗第二、第三和第四转的细胞中，在5 - 12,000Hz测量的运动反应的频率依赖性具有低通滤波器特性。然而，每个外毛细胞均不存在运动反应的频率调谐，并且未观察到不同转之间的系统性差异。
