Fini M, Giavaresi G, Spighi M, Giardino R, Florio M L
Depart. of Experimental Surgery, Rizzoli Orthopaedic Institutes, Bologna, Italy.
Artif Cells Blood Substit Immobil Biotechnol. 1995;23(1):101-8. doi: 10.3109/10731199509117671.
Haemoperfusion treatments have found clinical applications in various pathologies, even though problems due to the insufficient biocompatibility of sorbents have often limited its employment. Among these problems, there is that of heparin adsorption which can determine coagulation disorders or the necessity of a dosage correction during the extra corporeal circulation. The aim of the study was to test the influence of different methods of coating on heparin adsorption from an anion exchange resin coated with poly-hydroxyethyl-methacrylate. Eight haemoperfusion cartridges were prepared using 8 g of resin for each (4 cartridges contained resin coated by spray-coating technique and 4 coated by phase-separation one). Haemoperfusion was performed by using 600 IU of heparin (25,000 IU) corresponding to 0.12 ml in 500 ml of swine blood and a closed circuit. Blood flow rate was maintained at 15 ml/min and the circuit pressure was 70 torr during haemoperfusion. Blood samples were withdrawn at timed intervals before and after the column in order to evaluate blood heparin levels. The results demonstrated the superiority of the phase-separation technique that does not permit any interactions between adsorbent substance and drug. As a result of this, the adjustment of the dosage may not be necessary during further in vivo experiments.
血液灌流治疗已在各种病症中得到临床应用，尽管吸附剂生物相容性不足导致的问题常常限制了其应用。在这些问题中，存在肝素吸附问题，这可能会导致凝血障碍或在体外循环期间需要调整剂量。本研究的目的是测试不同涂层方法对涂有聚甲基丙烯酸羟乙酯的阴离子交换树脂吸附肝素的影响。制备了八个血液灌流柱，每个柱使用8克树脂（4个柱包含通过喷涂技术涂覆的树脂，4个柱通过相分离技术涂覆）。通过在500毫升猪血液中使用600国际单位肝素（25,000国际单位，相当于0.12毫升）并采用闭路循环进行血液灌流。血液灌流期间，血流速度维持在15毫升/分钟，回路压力为70托。在柱前后按时间间隔采集血样，以评估血液肝素水平。结果表明相分离技术具有优越性，该技术不会使吸附剂物质与药物之间发生任何相互作用。因此，在进一步的体内实验中可能无需调整剂量。
